Development of an S-G2 micronucleus assay for the detection of in vitro chromosomal radiosensitivity in high-risk women subjected to early mammography screening for breast cancer Universiteit Gent
Ionizing radiation induces DNA double-strand breaks (DSB). The two major repair pathways for DSB are homologous recombination (HR), operating only in late S and G2 phase, and non-homologous end joining (NHEJ), active throughout the whole cell cycle. It is known that the micronucleus (MN) assay, based on irradiation of cells in the G0 phase of the cycle cannot unequivocally detect enhanced radiosensitivity in carriers of BRCA1 and BRCA2 ...