Publicaties
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Roles of distal aspartate and arginine of B-class dye-decolorizing peroxidase in heterolytic hydrogen peroxide cleavage Universiteit Antwerpen
Dye-decolorizing peroxidases (DyPs) represent the most recently classified hydrogen peroxide-dependent heme peroxidase family. Although widely distributed with more than 5000 annotated genes and hailed for their biotechnological potential, detailed biochemical characterization of their reaction mechanism remains limited. Here, we present the high-resolution crystal structures of WT B-class DyP from the pathogenic bacterium Klebsiella pneumoniae ...
Electrochemical determination of hydrogen peroxide using **Rhodobacter capsulatus** cytochrome c peroxidase at a gold electrode Universiteit Antwerpen
We describe the redox behaviour of horse heart cytochrome c (HHC) and Rhodobacter capsulatus cytochrome c peroxidase (RcCCP) at a gold electrode modified with 4,4′-bipyridyl. RcCCP shows no additional oxidation or reduction peaks compared to the electrochemistry of only HHC, which indicates that it most likely binds to HHC and results in a potential downshift of the voltammetric signals of the latter. Furthermore, the electrochemical ...
The role of singlet oxygen, superoxide, hydroxide, and hydrogen peroxide in the photoelectrochemical response of phenols at a supported highly fluorinated zinc phthalocyanine Universiteit Antwerpen
Photoelectrochemical (PEC) sensing of phenolic compounds using singlet oxygen (1O2)-generating photocatalysts has emerged as a powerful detection tool. However, it is currently not known how experimental parameters, such as pH and applied potential, influence the generation of reactive oxygen species (ROS) and their photocurrents. In this article, the PEC response was studied over the 6 to 10 pH range using a rotating (ring) disk (R(R)DE) set-up ...
Electrochemical determination of hydrogen peroxide using Rhodobacter capsulatus cytochrome c peroxidase at a gold electrode Universiteit Gent
Uranium exposure induces nitric oxide and hydrogen peroxide generation in Arabidopsis thaliana Universiteit Hasselt
Uranium (U) is a nonessential and toxic heavy metal and radioactive element. Nitric oxide (NO) and hydrogen peroxide (H2O2) are intimately involved in the growth, development and metabolic function of plant cells. In addition to possible toxic activities, a role for these signalling molecules in the regulation of plant responses to toxic metals has been proposed. Here, we investigated the synthesis of NO and H2O2 in Arabidopsis thaliana ...
Detection of hydrogen peroxide vapor by use of manganese(IV) oxide as catalyst for calorimetric gas sensors Universiteit Hasselt
In this work, the catalyst manganese(IV) oxide (MnO2), of calorimetric gas sensors (to monitor the sterilization agent vaporized hydrogen peroxide) has been investigated in more detail. Chemical analyses by means of X-ray-induced photoelectron spectroscopy have been performed to unravel the surface chemistry prior and after exposure to hydrogen peroxide vapor at elevated temperature, as applied in the sterilization processes of beverage cartons. ...
Towards a multi-sensor system for the evaluation of aseptic processes employing hydrogen peroxide vapour (H(2)O(2)) Universiteit Hasselt KU Leuven
The presented work focuses on the development of a multi-sensor system for the evaluation of aseptic processes employing hydrogen peroxide vapour. In a first step, selected commercially available gas sensors have been investigated on cross-sensitivity towards hydrogen peroxide vapour and humidity. An MOX (TGS 816, Figaro) and a solid-electrolyte gas sensor (SO-A0-250, Electrovac) (lambda-probe) have shown good characteristics in terms of ...
Inactivation of murine norovirus 1, coliphage phiX174, and Bacteroides [corrected] fragilis phage B40-8 on surfaces and fresh-cut iceberg lettuce by hydrogen peroxide and UV light Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent KU Leuven
In this study, the inactivating properties of liquid hydrogen peroxide (L-H(2)O(2)), vaporized hydrogen peroxide (V-H(2)O(2)), UV light, and a combination of V-H(2)O(2) and UV light were tested on murine norovirus 1 (MNV-1) and bacteriophages (fX174 and B40-8) as models for human noroviruses. Disinfection of surfaces was examined on stainless steel discs based on European Standard EN 13697 (2001). For fresh-produce decontamination, a mixture of ...
Hydrogen Peroxide, Signaling in Disguise during Metal Phytotoxicity Universiteit Hasselt
Plants exposed to excess metals are challenged by an increased generation of reactive oxygen species (ROS) such as superoxide (O-2(center dot-)), hydrogen peroxide (H2O2) and the hydroxyl radical ((OH)-O-center dot). The mechanisms underlying this oxidative challenge are often dependent on metal-specific properties and might play a role in stress perception, signaling and acclimation. Although ROS were initially considered as toxic compounds ...