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Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells Vrije Universiteit Brussel
Abstract
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells Universiteit Gent
The use of human embryonic stem cells (hESC) in both research and therapeutic applications requires relatively large homogeneous populations of differentiated cells. The differentiation of three hESC lines into highly homogeneous populations of osteoprogenitor-like (hESC-OPL) cells is reported here. These cells could be expanded in a defined culture system for more than 18 passages, and showed a fibroblast-like morphology and a normal stable ...
Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells. Vrije Universiteit Brussel
The use of human embryonic stem cells (hESC) in both research and therapeutic applications requires relatively large homogeneous populations of differentiated cells. The differentiation of three hESC lines into highly homogeneous populations of osteoprogenitor-like (hESC-OPL) cells is reported here. These cells could be expanded in a defined culture system for more than 18 passages, and showed a fibroblast-like morphology and a normal stable ...
Activin A-derived human embryonic stem cells show increased competence to differentiate into primordial germ cell-like cells Universiteit Gent
Derivation of human embryonic stem cells using a post-inner cell mass intermediate Universiteit Gent
Uncovering low-level mosaicism in human embryonic stem cells using high throughput single cell shallow sequencing Universiteit Gent Vrije Universiteit Brussel
Human pluripotent stem cells (hPSCs) have significant levels of low-grade genetic mosaicism, which commonly used techniques fail to detect in bulk DNA. These copy number variations remain a hurdle for the clinical translation of hPSC, as their effect in vivo ranges from unknown to dangerous, and the ability to detect them will be necessary as the field advances. As such there is need for techniques which can efficiently analyse genetic content ...
Efficient derivation of mesenchymal stem cells from human embryonic stem cells for tissue engineering Universiteit Gent
Increased primordial germ cell (PGC) specification from human embryonic stem cells (hESC) derived in Activin A Universiteit Gent
Recent advances and prospects in the differentiation of pancreatic cells from human embryonic stem cells Vrije Universiteit Brussel
Recent studies with human embryonic stem (hES) cells have established new protocols for substantial generation of pancreatic progenitors from definitive endoderm. These findings add to the efficient derivation of definitive endoderm, which is controlled by Wnt and Nodal pathways, and delineate a step forward in the quest for alternative beta cell sources. It also indicates that critical refining of the available strategies might help define a ...