Publicaties
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Improved biosynthesis and characterization of silver nanoparticles using Laggera crispata (Vahl) Hepper and J.R.l Wood leaves extract Universiteit Gent
HiPlex amplicon sequencing to identify CRISPR/Cas edits in Cichorium Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Analytical aspects of the antinuclear antibody test by HEp-2 indirect immunofluorescence: EFLM report on an international survey KU Leuven
OBJECTIVES: Detection of antinuclear antibodies (ANA) by indirect immunofluorescence assay using HEp-2 cells (HEp-2 IFA) is used to screen for various autoimmune diseases. HEp-2 IFA suffers from variability, which hampers harmonization. METHODS: A questionnaire was developed to collect information on HEp-2 IFA methodology, computer-assisted diagnosis (CAD) systems, training, inter-observer variability, quality assessment, reagent lot change ...
Anti-Ki/anti-PA28γ autoantibodies contribute to the HEp-2 indirect immunofluorescence nuclear speckled pattern KU Leuven
OBJECTIVES: Antinuclear antibodies (ANAs) are associated with several autoimmune diseases. Indirect immunofluorescence (IIF) on human epithelial type 2 (HEp-2) cells is the golden standard for ANA detection in the clinic. In case of a positive HEp-2 IIF test result, follow-up tests are done to determine autoantibody specificity. For a fraction of the HEp-2 IIF-positive samples, the nature of the autoantigens remains uncharacterized. Our ...
HEp-2 Cell pattern segmentation for the support of autoimmune disease diagnosis Universiteit Gent
The Indirect Immune Fluorescence Test (iIFT) is the most commonly used screening method for the diagnosis of autoimmune diseases. The presence of certain autoimmune diseases is proven by immunologically detecting their corresponding auto-antibodies using the HEp-2 cancer cell line. For this purpose HEp-2 cells are added to the patients' blood serum containing certain auto-antibodies which will bond with the HEp-2 cells leading to a wide variety ...
HEp-2 cell pattern segmentation for the support of autoimmune disease diagnosis Universiteit Antwerpen
Hydrophilic interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer KU Leuven Universiteit Gent
HEPES is a zwitterionic buffer component used as a raw material in the GMP-manufacturing of advanced therapy medicinal products (ATMPs), hence requiring an adequate assay method with sufficient selectivity toward related impurities. Therefore, a hydrophilic interaction chromatography (HILIC) method was developed. Different factors were investigated towards the retention behavior of HEPES, its analogue EPPS and its starting material isethionate: ...