Publicaties
Gekozen filters:
Gekozen filters:
Toward safe genetically modified organism through the chemical diversification of nucleic acids KU Leuven
Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs) Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a % of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. We introduced a ...
Molecular toolbox for the identification of unknown genetically modified organisms Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent
Competent laboratories monitor genetically modified organisms (GMOs) and products derived thereof in the food and feed chain in the framework of labeling and traceability legislation. In addition, screening is performed to detect the unauthorized presence of GMOs including asynchronously authorized GMOs or GMOs that are not officially registered for commercialization (unknown GMOs). Currently, unauthorized or unknown events are detected by ...
Coherence between legal requirements and approaches for detection of genetically modified organisms (GMOs) and their derived products Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Analytical methods for the qualitative and quantitative detection of genetically modified (GM) products may serve multiple purposes. Legal requirements differ among jurisdictions, ranging from no requirements to mandatory use of event-specific quantitation and implementation of production chain traceability. Although efforts have been taken to harmonize the analytical methodology at national, regional, and international levels, no normative ...
The role of knowledge in student opinions on genetically modified organisms : an intervention study Universiteit Gent
Biotechnology, which includes genetic modification (GM), is often considered to be a controversial topic. Large amounts of factual information on GM as well as opinions are widely spread, which can affect people’s opinions and attitudes towards GM. This paper aims to investigate the nature of students’ acquisition of new factual information about genetically modified organisms (GMOs), and how this can be related to how they cope with new factual ...
Belgian dietitians′ knowledge, perceptions and willingness-to-recommend of genetically modified food and organisms Universiteit Gent
BackgroundDietitians play a critical role in the public's relationship with food and are often overlooked as an important stakeholder group in the general debate about sustainable food. Genetically modified organisms (GMOs) are one type of modern food source that could contribute to a more sustainable food system. This case study is the first to examine the knowledge, perception and willingness-to-recommend (WTR) genetically modified (GM) foods ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent KU Leuven
Because the number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several pioneers already investigated Next Generation Sequencing (NGS) as an alternative to qPCR, its practical use has not been assessed for routine analysis. In this study a statistical framework was developed to predict the number of NGS ...
Detecting un-authorized genetically modified organisms (GMOs) and derived materials Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Genetically modified plants, in the following referred to as genetically modified organisms or GMOs, have been commercially grown for almost two decades. In 2010 approximately 10% of the total global crop acreage was planted with GMOs (James, 2011). More than 30 countries have been growing commercial GMOs, and many more have performed field trials. Although the majority of commercial GMOs both in terms of acreage and specific events belong to ...
Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
An event-specific PCR method for detection and
quantification of genetically modified Roundup Ready soybean
(RRS) is described in this article. The complete DNA
sequence at both the right and left integration sites of this
genetically modified organism has recently been determined.
Based on these sequence data, transformation eventspecific
primer pairs were developed. These primers amplify
a fragment of the ...
quantification of genetically modified Roundup Ready soybean
(RRS) is described in this article. The complete DNA
sequence at both the right and left integration sites of this
genetically modified organism has recently been determined.
Based on these sequence data, transformation eventspecific
primer pairs were developed. These primers amplify
a fragment of the ...