Publicaties
Contribution of postnatally formed small beta cell aggregates to functional beta cell mass in adult rat pancreas Vrije Universiteit Brussel
METHODS: Conditions were defined for (1) counting total beta cell number in pancreases with relative error of
RESULTS: Pancreases of 10-week-old male Wistar rats contained ...
Direct effect of glucocorticoids on glucose-activated adult rat beta cells increases their cell number and their functional mass for transplantation Vrije Universiteit Brussel
Compounds that increase beta cell number can serve beta cell replacement therapies in diabetes. In vitro studies have identified several agents that can activate DNA synthesis in primary beta cells, however in small percentages of cells and without demonstration of increases in cell number. We used whole-well multi-parameter imaging to first screen a library of 1280 compounds for their ability to recruit adult rat beta cells into DNA ...
Functional beta cell mass from device-encapsulated hESC-derived pancreatic endoderm achieving metabolic control Vrije Universiteit Brussel
Human stem cells represent a potential source for implants that replace the depleted functional beta cell mass (FBM) in diabetes patients. Human embryonic stem cell-derived pancreatic endoderm (hES-PE) can generate implants with glucose-responsive beta cells capable of reducing hyperglycemia in mice. This study with device-encapsulated hES-PE (4 × 10 6 cells/mouse) determines the biologic characteristics at which implants establish metabolic ...
Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice Vrije Universiteit Brussel
A combination of cytokines EGF and CNTF protects the functional beta cell mass in mice with short-term hyperglycaemia Vrije Universiteit Brussel
AIMS/HYPOTHESIS: When the beta cell mass or function declines beyond a critical point, hyperglycaemia arises. Little is known about the potential pathways involved in beta cell rescue. As two cytokines, epidermal growth factor (EGF) and ciliary neurotrophic factor (CNTF), restored a functional beta cell mass in mice with long-term hyperglycaemia by reprogramming acinar cells that transiently expressed neurogenin 3 (NGN3), the current study ...
Functional beta cell mass and insulin sensitivity is decreased in insulin-independent pancreas-kidney recipients KU Leuven
Assessment of functional beta cell mass in macro-encapsulated stem cell-generated implants Vrije Universiteit Brussel
Glucose regulates rat beta cell number through age-dependent effects on beta cell survival and proliferation. Vrije Universiteit Brussel
Glucose effects on beta cell survival and DNA-synthesis suggest a role as regulator of beta cell mass but data on beta cell numbers are lacking. We examined outcome of these influences on the number of beta cells isolated at different growth stages in their population.
METHODS:
Beta cells from neonatal, young-adult and old rats were cultured serum-free for 15 days. Their number was counted by automated ...
Pre-existing insulin autoantibodies predict efficacy of otelixizumab in preserving residual beta cell function in recent-onset type 1 diabetes Vrije Universiteit Brussel
Immune intervention trials in recent-onset type 1 diabetes would benefit from biomarkers associated with good therapeutic response. In the previously reported randomized placebo-controlled anti-CD3 study (otelixizumab; GlaxoSmithKline), we tested the hypothesis that specific diabetes autoantibodies might serve this purpose.
RESEARCH DESIGN AND METHODS:
In the included patients (n = 40 otelixizumab, n = 40 ...