Publicaties
Gekozen filters:
Gekozen filters:
Structural and Enzymatic Characterization of ABgp46, a Novel Phage Endolysin with Broad Anti-Gram-Negative Bacterial Activity KU Leuven
The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an ...
Effects of AC-electrolysis on the enzymatic activity of glucose oxidase KU Leuven
The change in the activity of glucose oxidase subjected to an asymmetrical alternating current (AC) electric field is investigated via horseradish peroxidase (HRP)-coupled bioassay. The effect of the amplitude, frequency and enzyme concentration have been shown to affect the enzyme activity towards glucose oxidation. The decrease in the enzyme activity is directly related to the change in pH and temperature of the GOx solution during AC ...
Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana KU Leuven
Trehalose metabolism has profound effects on plant growth and metabolism, but the mechanisms involved are unclear. In Arabidopsis, 21 putative trehalose biosynthesis genes are classified in three subfamilies based on their similarity with yeast TPS1 (encoding a trehalose-6-phosphate synthase, TPS) or TPS2 (encoding a trehalose-6-phosphate phosphatase, TPP). Although TPS1 (Class I) and TPPA and TPPB (Class III) proteins have established TPS and ...
Enzymatic Desymmetrization of 19-nor-Vitamin D3 A-Ring Synthon Precursor: Synthesis, Structure Elucidation, and Biological Activity of 1α,25-Dihydroxy-3-epi-19-nor-vitamin D3 and 1β,25-Dihydroxy-19-nor-vitamin D3 KU Leuven
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim In a search for novel vitamin D derivatives of potential therapeutic value, structurally simple but synthetically challenging A-ring epimers of the 19-nor-Calcitriol [19-nor-1α,25-(OH)2-D3] at C1 and C3 were efficiently synthesized. Both analogues (1-epi- and 3-epi-19-nor-Calcitriol) were obtained through a convergent synthesis starting from cis,cis-1,3,5-cyclohexanetriol and the protected ...