On the occurrence of very low intra-particle diffusion rates in zwitterionic hydrophilic interaction liquid chromatography polymer columns Vrije Universiteit Brussel KU Leuven
Intra-particle diffusion is investigated in particle-packed and monolithic zwitterionic hydrophilic interaction liquid chromatography (HILIC) polymer columns. For this purpose, plate heights are first measured over a broad range of velocities for polar compounds with zone retention factors between k″ = 2 and 10 on all column types, multiplied with the corresponding velocity, and extrapolated to zero velocity. The thus obtained B-term ...