An apparatus for detecting fluorescent light emitted from a sample, a biosensor system, and a detector for detecting supercritical angle fluorescent light Interuniversitair Micro-Electronica Centrum vzw
Current surface sensitivity techniques which involve evanescent excitation of fluorophores have been around for a long time. Evanescent excitation of fluorophores provides better signal to noise ratio than trans-illumination for real-time measurements, where the bulk solution contains a large concentration of fluorophores. However, this requires either total internal reflection fluorescence microscopy (TIRF), which is a bulky, expensive technique or waveguides that are quite expensive to manufacture. One of the promising techniques that has been shown to improve selectivity towards surface ...