Publicaties
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Female human pluripotent stem cells rapidly lose X chromosome inactivation marks and progress to a skewed methylation pattern during culture Vrije Universiteit Brussel
STUDY HYPOTHESIS:
Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation?
STUDY FINDING:
We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapid loss of histone H3 lysine 27 trimethylation (H3K27me3) and long non-coding X-inactive ...
Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation?
STUDY FINDING:
We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapid loss of histone H3 lysine 27 trimethylation (H3K27me3) and long non-coding X-inactive ...
X chromosome inactivation in human pluripotent stem cells as a model for human development: back to the drawing board? Vrije Universiteit Brussel
BACKGROUND:
Human pluripotent stem cells (hPSC), both embryonic and induced (hESC and hiPSC), are regarded as a valuable in vitro model for early human development. In order to fulfil this promise, it is important that these cells mimic as closely as possible the in vivo molecular events, both at the genetic and epigenetic level. One of the most important epigenetic events during early human development is X chromosome inactivation ...
Human pluripotent stem cells (hPSC), both embryonic and induced (hESC and hiPSC), are regarded as a valuable in vitro model for early human development. In order to fulfil this promise, it is important that these cells mimic as closely as possible the in vivo molecular events, both at the genetic and epigenetic level. One of the most important epigenetic events during early human development is X chromosome inactivation ...
Chromosome fragility at FRAXA in human cleavage stage embryos at risk for fragile X syndrome Vrije Universiteit Brussel
Fragile X syndrome (FXS), the most common inherited intellectual disability syndrome, is caused by expansion and hypermethylation of the CGG repeat in the 5' UTR of the FMR1 gene. This expanded repeat, also known as the rare fragile site FRAXA, causes X chromosome fragility in cultured cells from patients but only when induced by perturbing pyrimidine synthesis. We performed preimplantation genetic diagnosis (PGD) on 595 blastomeres biopsied ...
LPS resistance of SPRET/Ei mice is mediated by Gilz, encoded by the Tsc22d3 gene on the X chromosome Universiteit Gent
X Chromosome Dosage Modulates Multiple Molecular and Cellular Properties of Mouse Pluripotent Stem Cells Independently of Global DNA Methylation Levels KU Leuven
Reprogramming female mouse somatic cells into induced pluripotent stem cells (iPSCs) leads to X-chromosome reactivation. The extent to which increased X- chromosome dosage (X-dosage) in female iPSCs compared with male iPSCs leads to differences in the properties of iPSCs is still unclear. We show that chromatin accessibility in mouse iPSCs is modulated by X-dosage. Specific sets of transcriptional regulator motifs are enriched in chromatin with ...
Restricted X chromosome introgression and support for Haldane's rule in hybridizing damselflies KU Leuven
Contemporary hybrid zones act as natural laboratories for the investigation of species boundaries and may shed light on the little understood roles of sex chromosomes in species divergence. Sex chromosomes are considered to function as a hotspot of genetic divergence between species; indicated by less genomic introgression compared to autosomes during hybridization. Moreover, they are thought to contribute to Haldane's rule, which states that ...
Evidence for faster X chromosome evolution in spiders Universiteit Gent
Structural and numerical changes of chromosome X in patients with esophageal atresia Universiteit Gent
Uncovering the epigenetic landscape of cell fate reprogramming and X chromosome reactivation KU Leuven
In this thesis, I aimed to define the dynamics and the mechanisms that orchestrate the reversal of stable gene silencing during reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). I used X chromosome reactivation as a paradigm to better understand the reversal of silent chromatin. In addition, my goal was also to define the gene regulatory logic of mammalian pre- implantation development and reprogramming, as well as to ...