Publicaties
In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors Vrije Universiteit Brussel
The myelin sheath is essential for the rapid and efficient propagation of action potentials. However, our understanding of the basic molecular mechanisms that regulate myelination, demyelination and remyelination is limited. Schwann cells produce myelin in the peripheral nervous system and remain associated with the axons of peripheral neurons throughout axonal migration to the target. Owing to the intimate relationship between these cell ...
In Vivo Introduction of Transgenes into Mouse Sciatic Nerve Cells Using Viral Vectors Vrije Universiteit Brussel
Myelinated fibers are essential for the rapid and efficient propagation of nerve information throughout the body. These fibers result from an intimate crosstalk between myelinating glia and the myelinated axons and, because it is difficult to fully reproduce these interactions in vitro, the basic molecular mechanisms that regulate myelination, demyelination, and remyelination remain unclear. Schwann cells produce myelin in the peripheral ...
Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture Universiteit Gent
Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing Universiteit Gent
Chicken beta-globin insulator overcomes variegation of transgenes in Xenopus embryos Universiteit Gent
Characterization of position-induced spatial and temporal regulation of transgene promoter activity in plants Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Quantitative differences in transgene expression between independent transformants are generally ascribed to different integration sites of the transgene (position effect). The contribution of spatial and temporal changes in transgene promoter activity to these position-induced differences in transgene expression in planta are characterized, using the firefly luciferase (luc) reporter system. The activity of three different promoters ...
Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells Vrije Universiteit Brussel
Achieving transgene integration into preselected genomic sites is currently one of the central tasks in stem cell gene therapy. A strategy to mediate such targeted integration involves site-specific endonucleases. Two genomic sites within the MBS85 and chemokine (C-C motif) receptor 5 (CCR5) genes (AAVS1 and CCR5 zinc-finger nuclease (CCR5-ZFN) sites, respectively) have recently been suggested as potential target regions for integration as ...