A thermodynamic model of the cooperative interaction between the archaeal transcription factor Ss-LrpB and its tripartite operator DNA Vrije Universiteit Brussel
Ss-LrpB is a transcription factor of the archaeon Sulfolobus solfataricus that belongs to the Leucine-responsive Regulatory Protein family. This protein binds to three distinct binding sites in the control region of its own gene, suggestive of autoregulation. Here, we present a detailed study of the thermodynamic and conformational rules that govern the interaction between Ss-LrpB and its tripartite operator DNA. Lane-per-lane partition analysis ...