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Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis Universiteit Gent
Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp(gt/gt )mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born normal, but soon develops chronic, mild hepatocyte injury, ...
Gene Signatures Detect Damaged Liver Sinusoidal Endothelial Cells in Chronic Liver Diseases Vrije Universiteit Brussel
Liver sinusoidal endothelial cells have a gatekeeper function in liver homeostasis by permitting substrates from the bloodstream into the space of Disse and regulating hepatic stellate cell activation status. Maintenance of LSEC's highly specialized phenotype is crucial for liver homeostasis. During liver fibrosis and cirrhosis, LSEC phenotype and functions are lost by processes known as capillarization and LSEC dysfunction. LSEC ...
The role of liver sinusoidal cells in hepatocyte-directed gene transfer KU Leuven
Hepatocytes are a key target for gene therapy of inborn errors of metabolism as well as of acquired diseases such as liver cancer and hepatitis. Gene transfer efficiency into hepatocytes is significantly determined by histological and functional aspects of liver sinusoidal cells. On the one hand, uptake of vectors by Kupffer cells and liver sinusoidal endothelial cells may limit hepatocyte transduction. On the other hand, the presence of ...
Functionality based method for simultaneous isolation of rodent hepatic sinusoidal cells Vlaams Instituut voor Biotechnologie Vrije Universiteit Brussel KU Leuven
Human liver sinusoidal endothelial cells but not hepatocytes contain factor VIII KU Leuven
BACKGROUND: Although the liver is the major site of coagulation factor VIII (FVIII) synthesis, the type of cells producing FVIII within the liver is still unclear. OBJECTIVES: To measure FVIII in extracts of primary liver sinusoidal endothelial cells (LSECs) and hepatocytes, thereby preventing potential bias resulting from the modifications of the cell phenotype that can take place during in vitro culture. METHODS: LSECs were purified by flow ...
Rapid clearance of the circulating metastatic factor autotaxin by the scavenger receptors of liver sinusoidal endothelial cells KU Leuven
Autotaxin, also known as NPP2 (nucleotide pyrophosphatase/phosphodiesterase 2), is a secreted lysophospholipase-D that generates lysophosphatidic acid and thereby promotes the metastatic and invasive properties of tumor cell as well as angiogenesis. We show here that, in mice, NPP2 is cleared from the circulation within minutes and is retained by the liver sinusoidal endothelial cells (LSECs). The binding of NPP2 to isolated LSECs resulted in ...
Systemic antigen cross-presented by liver sinusoidal endothelial cells induces liver-specific CD8 T-cell retention and tolerization. Vrije Universiteit Brussel
Unraveling the determinants of liver sinusoidal endothelial cell features during health and disease - The context-dependent role of ZEB2 KU Leuven
Liver sinusoidal endothelial cells (LSECs) are a highly specialized type of endothelial cells (ECs) characterized by a high permeability due to the presence of transcellular pores, called fenestrations, a poorly developed basement membrane, and high expression levels of scavenger receptors. Due to this high permeability and their position at the interface between blood and hepatocytes, LSECs act as a selective filter of the blood, allowing ...