Publicaties
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Computational design of symmetric proteins: rounding up evolution of the propeller proteins KU Leuven
The host laboratory recently made 6-fold and 8-fold symmetric circular proteins using computational biomolecular modelling techniques. The 6-fold symmetric protein called Pizza has promising properties for use in nano-technology. This Pizza protein was also redesigned to coordinate metal ions, thus forming the smallest nanocrystal reported to this date. In this project, I will aim to design other symmetric proteins with a different rotational ...
Structure and function analysis of two Early Response to Dehydration Proteins: intrinsically disordered proteins preventing the collapse of the proteostasis network during abiotic stress in plants Vrije Universiteit Brussel
Rab proteins and Rab-associated proteins: major actors in the mechanism of protein-trafficking disorders KU Leuven
Ras-associated binding (Rab) proteins and Rab-associated proteins are key regulators of vesicle transport, which is essential for the delivery of proteins to specific intracellular locations. More than 60 human Rab proteins have been identified, and their function has been shown to depend on their interaction with different Rab-associated proteins regulating Rab activation, post-translational modification and intracellular localization. The ...
ELISA based detection of soybean proteins: a comparative study using antibodies against modified and native protein Universiteit Gent
Effect of protein glycation in the presence or absence of wheat proteins on detection of soybean proteins by commercial ELISA Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent
Soybean (Glycine max) is the world's primary provider of protein and oil and is widely used in foodstuffs. However, the use of soybean in foodstuffs might pose a serious threat to allergic consumers since some proteins can cause allergic reactions. To date mostly ELISA methods are used for testing contamination of foodstuffs with soybean. In view of the complexity regarding allergen detection in foodstuffs and appropriate food product labelling, ...
Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1β and Interferon-γ in Liver Bystander Cells KU Leuven
Chronic hepatitis C virus (HCV) infection accounts for a large proportion of hepatic fibrosis and carcinoma cases observed worldwide. Mechanisms involved in HCV-induced hepatic injury have yet to be fully elucidated. Of particular interest is the capacity of HCV to regulate inflammatory responses. Here, we reveal modulation of cytokine activity by the HCV proteins non-structural protein 3 (NS3), glycoprotein E2, and core protein for their ...
Endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection KU Leuven Universiteit Gent Universiteit Antwerpen
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. During the streptavidin-based detection of glycoproteins in Lactobacillus rhamnosus GG with biotinylated lectin probes, a strong positive band of approximately 125 kDa was observed, present in different cellular fractions. This potential glycoprotein reacted heavily with concanavalin A (ConA), ...
Bacterial Obg proteins: GTPases at the nexus of protein and DNA synthesis KU Leuven
Obg proteins (also known as ObgE, YhbZ and CgtA) are conserved P-loop GTPases, essential for growth in bacteria. Like other GTPases, Obg proteins cycle between a GTP-bound ON and a GDP-bound OFF state, thereby controlling cellular processes. Interestingly, the in vitro biochemical properties of Obg proteins suggest that they act as sensors for the cellular GDP/GTP pools and adjust their activity according to the cellular energy status. Obg ...
Protein Interface Pharmacophore Mapping Tools for Small Molecule Protein: Protein Interaction Inhibitor Discovery KU Leuven
Protein:protein interactions are becoming increasingly significant as potential drug targets; however, the rational identification of small molecule inhibitors of such interactions remains a challenge. Pharmacophore modelling is a popular tool for virtual screening of compound libraries, and has previously been successfully applied to the discovery of enzymatic inhibitors. However, the application of pharmacophore modelling in the field of ...