Site-specific protein functionalization by expressed protein ligation for advanced biomaterials Universiteit Hasselt
In situ monitoring and optimization of CuAAC-mediated protein functionalization of biosurfaces Universiteit Hasselt
Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation Vrije Universiteit Brussel
An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne functionality at their C-terminus via the intein-mediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle® T7 cells, in the commonly used Luria-Bertani (LB) medium. The combination of these factors results in a ...