Publicaties
Gekozen filters:
Gekozen filters:
Thiol-Disulfide Exchange in Signaling: Disulfide Bonds as a Switch Vrije Universiteit Brussel
The major function of disulfide bonds is not only the stabilization of protein structures. Over the last 30 years, a change in perspective took place driven by groundbreaking experiments, which promoted disulfide bonds to central players in essential thiol disulfide exchange reactions involved in signal transduction, thiol protection and redox homeostasis regulation. This new view stimulated redox research and led to the discovery of novel redox ...
Internal Disulfide Bonding and Glycosylation of Interleukin-7 Protect Against Proteolytic Inactivation by Neutrophil Metalloproteinases and Serine Proteases KU Leuven
Interleukin 7 (IL-7) is a cell growth factor with a central role in normal T cell development, survival and differentiation. The lack of IL-7-IL-7 receptor(R)-mediated signaling compromises lymphoid development, whereas increased signaling activity contributes to the development of chronic inflammation, cancer and autoimmunity. Gain-of-function alterations of the IL-7R and the signaling through Janus kinases (JAKs) and signal transducers and ...
Modulation of Calcium Signaling of Angiotensin AT1, Endothelin ETA, and ETB Receptors by Silibinin, Quercetin, Crocin, Diallyl Sulfides, and Ginsenoside Rb1 Vrije Universiteit Brussel
Angiotensin II and endothelin-1 are potent vasoconstrictive peptides that play a central role in blood pressure regulation. Both peptides exert their pleiotropic effects via binding to their respective G-protein-coupled receptors, i.e., angiotensin AT1 and endothelin type A and type B receptors. In the present study, we have selected six structurally different plant-derived compounds with known cardioprotective properties to evaluate their ...
The steroidal lactone withaferin A impedes T-cell motility by inhibiting the kinase ZAP70 and subsequent kinome signaling Universiteit Antwerpen
The steroidal lactone withaferin A (WFA) is a dietary phytochemical, derived from Withania somnifera. It exhibits a wide range of biological properties, including immunomodulatory, anti-inflammatory, antistress, and anticancer activities. Here we investigated the effect of WFA on T-cell motility, which is crucial for adaptive immune responses as well as autoimmune reactions. We found that WFA dose-dependently (within the concentration range of ...
Thiol-disulfide redox switches for the enzymatic reduction of protein methionine sulfoxide Vrije Universiteit Brussel
During phagocytosis, the human immune system releases oxidants to damage the macromolecules of pathogenic bacteria. During this period of oxidative stress, oxidation leads to alterations in protein function, and influences downstream signaling pathways of the pathogen. To counteract, bacteria have developed different types of anti-oxidant defense systems to overcome oxidative stress. The main objective of my PhD thesis was to understand the ...
CysQuant : simultaneous quantification of cysteine oxidation and protein abundance using data dependent or independent acquisition mass spectrometry Universiteit Gent Vlaams Instituut voor Biotechnologie
Protein cysteinyl thiols are susceptible to reduction-oxidation reactions that can influence protein function. Accurate quantification of cysteine oxidation is therefore crucial for decoding protein redox regulation. Here, we present CysQuant, a novel approach for simultaneous quantification of cysteine oxidation degrees and protein abundancies. CysQuant involves light/heavy iodoacetamide isotopologues for differential labeling of reduced and ...
Protein engineering of the chemokine CCL20 prevents psoriasiform dermatitis in an IL-23-dependent murine model. KU Leuven
Psoriasis is a chronic inflammatory skin disease characterized by the infiltration of T cell and other immune cells to the skin in response to injury or autoantigens. Conventional, as well as unconventional, γδ T cells are recruited to the dermis and epidermis by CCL20 and other chemokines. Together with its receptor CCR6, CCL20 plays a critical role in the development of psoriasiform dermatitis in mouse models. We screened a panel of CCL20 ...
The quiescin sulfhydryl oxidase (hQSOX1b) tunes the expression of resistin-like molecule alpha (RELM-α or mFIZZ1) in a wheat germ cell-free extract Vrije Universiteit Brussel
Background: Although disulfide bond formation in proteins is one of the most common types of post-translational modifications, the production of recombinant disulfide rich proteins remains a challenge. The most popular host for recombinant protein production is Escherichia coli, but disulfide rich proteins are here often misfolded, degraded, or found in inclusion bodies.
Methodology/Principal findings: We optimize an in vitro wheat ...
Methodology/Principal findings: We optimize an in vitro wheat ...
Understanding the pK(a) of redox cysteines Vrije Universiteit Brussel
Many cellular functions involve cysteine chemistry via thiol-disulfide exchange pathways. The nucleophilic cysteines of the enzymes involved are activated as thiolate. A thiolate is much more reactive than a neutral thiol. Therefore, determining and understanding the pK(a)s of functional cysteines are important aspects of biochemistry and molecular biology with direct implications for redox signaling. Here, we describe the experimental and ...