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Investigating rapid diagnostic testing in Kenya's health system, 2018-2020: validating non-reporting in routine data using a health facility service assessment survey Instituut voor Tropische Geneeskunde

Angela K Moturi, Bibian N Robert, Felix Bahati, Peter Macharia, Emelda A Okiro

BACKGROUND: Understanding the availability of rapid diagnostic tests (RDTs) is essential for attaining universal health care and reducing health inequalities. Although routine data helps measure RDT coverage and health access gaps, many healthcare facilities fail to report their monthly diagnostic test data to routine health systems, impacting routine data quality. This study sought to understand whether non-reporting by facilities is due to ...

Clinical evaluation of BioFire® multiplex-PCR panel for acute undifferentiated febrile illnesses in travellers: a prospective multicenter study Instituut voor Tropische Geneeskunde

Daniel Camprubí-Ferrer, Ludovico Cobuccio, Steven Van Den Broucke, Leire Balerdi-Sarasola, Blaise Genton, Emmanuel Bottieau, Jessica Navero-Castillejos, Miguel J. Martinez, Corinne Jay, Anne Grange, et al.

BACKGROUND: Identifying the causes of Acute Undifferentiated Febrile Illness (AUFI) is key to improve the management of returning travellers with fever. We evaluated a BioFire®FilmArray® prototype panel of multiplex nucleic acid amplification tests (NAAT) targeting different relevant pathogens in travellers returning with fever.

METHODS: Prospective, multicentre study to evaluate a prototype panel in whole blood samples of adult ...

Accuracy of malaria diagnosis by clinical laboratories in Belgium Instituut voor Tropische Geneeskunde KU Leuven

Laura Loomans, Anali Conesa Botella, D'hondt, Jacob Verschueren, Dorien Van den Bossche, Marjan Van Esbroeck, Jan Jacobs

Background: The Belgian Reference Laboratory for Plasmodium offers a free-of-charge reference testing of malaria-positive or doubtful samples to clinical laboratories.

Methods: The final malaria diagnosis from the Reference Laboratory (microscopy, rapid diagnostic tests (RDTs) and Plasmodium species-specific PCR) were compared with the final diagnosis from peripheral Belgian laboratories. The Reference Laboratory reports were analysed ...

Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings Instituut voor Tropische Geneeskunde

Elisa Serra Casas, Paulo Manrique, Xavier C Ding, Gabriel Carrasco-Escobar, Freddy Alava, Anthony Gave, Hugo Rodriguez, Juan Contreras-Mancilla, Angel Rosas-Aguirre, Niko Speybroeck, et al.

BACKGROUND: Loop-mediated isothermal DNA amplification (LAMP) methodology offers an opportunity for point-of-care (POC) molecular detection of asymptomatic malaria infections. However, there is still little evidence on the feasibility of implementing this technique for population screenings in isolated field settings.

METHODS: Overall, we recruited 1167 individuals from terrestrial ('road') and hydric ('riverine') communities of the ...

Monkey malaria (Plasmodium knowlesi infection) after travelling to Thailand Instituut voor Tropische Geneeskunde

Inge Kroidl, Michael Seilmaier, Nicole Berens-Riha, Gisela Bretzel, Clemens Wendtner, Thomas Löscher

A case of malaria caused by Plasmodium knowlesi is described in a 52-year-old female German traveler after returning from Thailand. P. knowlesi is a parasite of macaques in Southeast Asia and has been recognized in recent years as an important and probably increasing cause of human malaria in some areas. At least 16 cases in international travelers have been published so far. This includes four cases imported to Germany. All German patients ...