The effect of apoptotic cells on virus-specific immune responses detected using IFN-gamma ELISPOT Universiteit Hasselt Universiteit Antwerpen
Interferon (IFN)-gamma ELISPOT can be used to monitor the magnitude of virus-specific cellular immune responses in vaccine trials. Often, IFN-gamma ELISPOT is performed with cryopreserved peripheral blood mononuclear cells (PBMC). However, it has not been well defined yet to what extent diminished cell viability of PBMC following cryopreseivation affects IFN-gamma responses in ELISPOT. Therefore, we assessed the influence of apoptotic cells on ...