Publicaties

1 - 10 of 66 resultaten

Zoek term:

Increase functional β-cell mass in subcutaneous alginate capsules with porcine prenatal islet cells but loss with human adult islet cells Vrije Universiteit Brussel

Ines De Mesmaeker, Thomas Robert, Krista Suenens, Freya Ophelie Van Hulle, Zhidong Ling, Peter Tomme, Daniel Jacobs-Tulleneers-Thevissen, Bart Keymeulen, Daniel Pipeleers

Alginate (Alg)-encapsulated porcine islet cell grafts are developed to overcome limitations of human islet transplantation. They can generate functional implants in animals when prepared from fetal, perinatal, and adult pancreases. Implants have not yet been examined for efficacy to establish sustained, metabolically adequate functional β-cell mass (FBM) in comparison with human islet cells. This study in immune-compromised mice demonstrates ...

Plasma GAD65, a Marker for Early beta-Cell Loss After Intraportal Islet Cell Transplantation in Diabetic Patients KU Leuven

Zhidong Ling, Pieter De Pauw, Daniel Jacobs-Tulleneers-Thevissen, Rui Mao, Pieter Gillard, Christiane S Hampe, Geert A Martens, Peter In't Veld, Ake Lernmark, Bart Keymeulen, et al.
CONTEXT AND OBJECTIVE: Intraportal islet transplantation can restore insulin production in type 1 diabetes patients, but its effect is subject to several interfering processes. To assess the influence of β-cell loss before and during engraftment, we searched for a real-time marker of β-cell destruction. Previous studies showed that 65-kDa isoform of glutamate decarboxylase (GAD65) is discharged by chemically damaged rat β-cells. We therefore ...

Hepatic steatosis after islet transplantation Vrije Universiteit Brussel

Lorenzo Piemonti

Percutaneous intra-portal islet transplantation (PIPIT) is a less invasive, safer, and repeatable therapeutic option for brittle type 1 diabetes, compared to surgical pancreas transplantation. Hepatic steatosis is a consequence of the islet engraftment but it is curiously present in a limited number of patients and its meaning is controversial. The aims of this study were to assess hepatic steatosis at ultrasound (US) after PIPIT ...

Relevance of cytotoxic alloreactivity under different immunosuppressive regimens in clinical islet cell transplantation Vrije Universiteit Brussel

Robert Hilbrands, Pieter Gillard, Bart Keymeulen, Daniel Pipeleers
Islet or beta cell transplantation provides a promising cure for type 1 diabetes patients, but insulin-independency decreases frequently over time. Immunosuppressive regimens are implemented attempting to cope with both auto- and alloimmunity after transplantation. We analysed the influence of different immunotherapies on autoreactive and alloreactive T cell patterns and transplant outcome. Patients receiving three different immunosuppressive ...

Relevance of cytotoxic alloreactivity under different immunosuppressive regimens in clinical islet cell transplantation KU Leuven

DL Roelen, VAL Huurman, R Hilbrands, Pieter Gillard, G Duinkerken, PWM van der Meer-Prins, MFJ Versteeg-Van der Voort Maarschalk, Chantal Mathieu, B Keymeulen, DG Pipeleers, et al.
Islet or beta cell transplantation provides a promising cure for type 1 diabetes patients, but insulin-independency decreases frequently over time. Immunosuppressive regimens are implemented attempting to cope with both auto- and alloimmunity after transplantation. We analysed the influence of different immunotherapies on autoreactive and alloreactive T cell patterns and transplant outcome. Patients receiving three different immunosuppressive ...

Salvage Islet Auto Transplantation After Relaparatomy Vrije Universiteit Brussel

BACKGROUND: To assess feasibility, safety, and metabolic outcome of islet auto transplantation (IAT) in patients undergoing completion pancreatectomy because of sepsis or bleeding after pancreatic surgery.

METHODS: From November 2008 to October 2016, approximately 22 patients were candidates to salvage IAT during emergency relaparotomy because of postpancreatectomy sepsis (n = 11) or bleeding (n = 11). Feasibility, efficacy, and safety ...

Calcineurin inhibitor-free immunosuppressive regimen in type 1 diabetes patients receiving islet transplantation Vrije Universiteit Brussel

BACKGROUND: Our final objective is to develop an adoptive therapy with tolerogenic donor-specific type 1 T regulatory cells for patients with type 1 diabetes undergoing islet transplantation. The achievement of this objective depends on the availability of an immunosuppressive treatment compatible with the survival, function, and expansion of type 1 T regulatory cells.

METHODS: For this purpose, we designed a single-group, phase 1 to 2 ...

Stem-Cell-Based Therapies for Improving Islet Transplantation Outcomes in Type 1 Diabetes KU Leuven

João Paulo Monteiro Carvalho Mori Cunha, Constantia Gysemans, Pieter Gillard, Chantal Mathieu
Beta-cell replacement by human islets or whole pancreas offers a life-saving therapeutic remedy for patients suffering from type 1 diabetes, providing considerable advantages with respect to diminishing total daily insulin dose and lowering frequencies of debilitating hypoglycemic reactions as well as preventing chronic micro- and macrovascular complications. Although remarkable progress has been made in this area, several hurdles remain, ...

Use of culture to reach metabolically adequate beta cell dose by combining donor islet cell isolates for transplantation in type 1 diabetes patients Vrije Universiteit Brussel

DaHae Lee, Pieter Gillard, Robert Hilbrands, Zhidong Ling, Ursule Van de Velde, Daniel Jacobs-Tulleneers-Thevissen, Geert Maleux, Bruno Lapauw, Laurent Crenier, Christophe De Block, et al.

BACKGROUND: Clinical islet transplantation is generally conducted within 72 hours after isolating sufficient beta cell mass. A preparation that does not meet the sufficient dose can be cultured until this is reached after combination with subsequent ones. This retrospective study examines whether metabolic outcome is influenced by culture duration.

METHOD: Forty type 1 diabetes recipients of intraportal islet cell grafts under ATG ...

Islet transplantation stabilizes hemostatic abnormalities and cerebral metabolism in individuals with type 1 diabetes Vrije Universiteit Brussel

OBJECTIVE Islets after kidney transplantation have been shown to positively affect the quality of life of individuals with type 1 diabetes (T1D) by reducing the burden of diabetes complications, but fewer data are available for islet transplantation alone (ITA). The aim of this study was to assess whether ITA has a positive impact on hemostatic and cerebral abnormalities in individuals with T1D. RESEARCH DESIGN AND METHODS Prothrombotic ...