Publicaties
Gekozen filters:
Gekozen filters:
Epigenetic regulation of gap junctional intercellular communication: More than a way to keep cells quiet? Vrije Universiteit Brussel
The establishment of gap junctional intercellular communication is a prerequisite for appropriate control of tissue homeostasis. Gap junctions consist of connexin proteins, whereby a myriad of factors govern the connexin life cycle. At the transcriptional level, most attention has yet been paid to the classical cis/trans machinery (i.e. the interaction between transcription factors and regulatory elements in connexin gene promoter regions) as a ...
Tracking changes in the histone modification pattern by untargeted mass spectrometry : how naïve are human embryonic stem cells and data-dependent acquisition? Universiteit Gent
In this dissertation, we profile the histone epigenome of hESCs during conversion from the primed to the naive state using an untargeted mass spectrometry (MS)-based approach. In total, 23 histone post-translational modifications (hPTMs) changed significantly over time. H3K27me3 was the most prominently increasing marker hPTM in naive hESCs. This is in line with what was recently described in mouse, prompting us to compare all the shared hPTM ...
Role of SIRT1 in Modulating Acetylation of the Sarco-Endoplasmic Reticulum Ca2+-ATPase in Heart Failure KU Leuven
RATIONALE: SERCA2a, sarco-endoplasmic reticulum Ca2+-ATPase, is a critical determinant of cardiac function. Reduced level and activity of SERCA2a are major features of heart failure. Accordingly, intensive efforts have been made to develop efficient modalities for SERCA2a activation. We showed that the activity of SERCA2a is enhanced by post-translational modification with SUMO1 (small ubiquitin-like modifier 1). However, the roles of other ...
Multifaceted functions of histone deacetylases in stress response Universiteit Gent
Histone deacetylases (HDACs) are important chromatin regulators essential for plant tolerance to adverse environments. In addition to histone deacetylation and epigenetic regulation, HDACs deacetylate non-histone proteins and thereby regu-late multiple pathways. Like other post-translational modifications (PTMs), acety-lation/deacetylation is a reversible switch regulating different cellular processes in plants. Here, by focusing on results ...
ZEB2 stably represses RAB25 expression through epigenetic regulation by SIRT1 and DNMTs during epithelial-to-mesenchymal transition Universiteit Gent
Background: Epithelial mesenchymal transition (EMT) is tightly regulated by a network of transcription factors (EMT-TFs). Among them is the nuclear factor ZEB2, a member of the zinc-finger E-box binding homeobox family. ZEB2 nuclear localization has been identified in several cancer types, and its overexpression is correlated with the malignant progression. ZEB2 transcriptionally represses epithelial genes, such as E-cadherin (CDH1), by directly ...
Histone deacetylase inhibition and the regulation of cell growth with particular reference to liver pathobiology. Vrije Universiteit Brussel
The transcriptional activity of genes largely depends on the accessibility of specific chromatin regions to transcriptional regulators. This process is controlled by diverse posttranscriptional modifications of the histone amino termini of which reversible acetylation plays a vital role. Histone acetyltransferases (HATs) are responsible for the addition of acetyl groups and
histone deacetylases (HDACs) catalyse the reverse reaction. In ...
histone deacetylases (HDACs) catalyse the reverse reaction. In ...
Synergetic effect of DNA demethylation and histone deacetylase inhibition in primary rat hepatocytes Vrije Universiteit Brussel
Both, DNA methylation and histone deacetylation play a crucial role in cancer development by silencing the expression of specific tumour suppressor genes. Several studies describe the use of combinations of DNA methyltransferase inhibitors (DNMT-i) and histone deacetylase inhibitors (HDAC-i) as an improved strategy to treat neoplasms. However, no information is available concerning their biological impact on healthy, non-malignant cells, ...
S-Nitrosylation of the histone deacetylase HDA19 stimulates its activity to enhance plant stress tolerance in Arabidopsis Universiteit Gent
Arabidopsis histone deacetylase HDA19 is required for gene expression programs of a large spectrum of plant developmental and stress-responsive pathways. How this enzyme senses cellular environment to control its activity remains unclear. In this work, we show that HDA19 is post-translationally modified by S-nitrosylation at 4 Cysteine (Cys) residues. HDA19 S-nitrosylation depends on the cellular nitric oxide level, which is enhanced under ...
Peptides as epigenetic modulators: therapeutic implications Universiteit Antwerpen Universiteit Gent
Peptides originating from different sources (endogenous, food derived, environmental, and synthetic) are able to influence different aspects of epigenetic regulation. Endogenous short peptides, resulting from proteolytic cleavage of proteins or upon translation of non-annotated out of frame transcripts, can block DNA methylation and hereby regulate gene expression. Peptides entering the body by digestion of food-related proteins can modulate DNA ...