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Occurrence and molecular characterization of cryptosporidium and giardia in captive wild ruminants in Belgium Koninklijke Maatschappij voor Dierkunde van Antwerpen Universiteit Gent
Both Cryptosporidium and Giardia are frequently found in the stool of domestic ruminants. especially young animals. Wild ruminants are also host to these protozoa, but the prevalence of these parasites in both free-ranging and captive nondomesticated ruminants needs to be further investigated. Moreover, the role of wild ruminants serving as reservoirs for these zoonotic parasites remains unclear. Therefore, a cross-sectional survey was conducted ...
High frequency of Cryptosporidium hominis infecting infants points to a potential anthroponotic transmission in Maputo, Mozambique Instituut voor Tropische Geneeskunde
Cryptosporidium is one of the most important causes of diarrhea in children less than 2 years of age. In this study, we report the frequency, risk factors and species of Cryptosporidium detected by molecular diagnostic methods in children admitted to two public hospitals in Maputo City, Mozambique. We studied 319 patients under the age of five years who were admitted due to diarrhea between April 2015 and February 2016. Single stool samples ...
Peri-parturient rise of Cryptosporidium oocysts in cows: new insights provided by duplex quantitative real-time PCR Instituut voor Tropische Geneeskunde
In order to clarify if a peri-parturient rise of Cryptosporidium parvum oocysts occurs in cows, faecal samples from 42 cows on two farms were collected. These samples were taken during the pre-parturient, the peri-parturient and the post-parturient periods. Two methods were used to detect the oocysts, a nested-PCR coupled with sequencing and a duplex real-time PCR (qPCR) that quantified Cryptosporidium spp. DNA concentration. The qPCR results ...
Age-stratified Bayesian analysis to estimate sensitivity and specificity of four diagnostic tests for the detection of Cryptosporidium spp. oocysts in neonatal calves Instituut voor Tropische Geneeskunde
There is no gold standard diagnostic test for the detection of bovine cryptosporidiosis. Infection is usually the highest in two-week-old calves and these calves also excrete high numbers of oocysts. These factors may give rise to variations in the sensitivity and specificity of the various diagnostic tests used to detect infection in calves of varying ages. An age-stratified Bayesian analysis was carried out to determine the optimum diagnostic ...
Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium Koninklijke Maatschappij voor Dierkunde van Antwerpen Universiteit Gent
Stool samples from Belgian patients Suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54.2% and C. parvum 45.8% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IlaA15G2R1 of C. parvum were ...