Publicaties
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Mini-Hydrocyclone Separation of Cyanobacterial and Green Algae: Impact on Cell Viability and Chlorine Consumption Universiteit Hasselt
The co-occurrence of non-toxic phytoplankton alongside cyanobacteria adds to the challenge of treating source waters with harmful algal blooms. The non-toxic species consume the oxidant and, thereby, reduce the efficacy of oxidation of both the extracellular and intracellular cyanotoxins. In this work, a 3D printed mini-hydrocyclone was used to separate a mixture of non-toxic green algae, Scenedesmus obliquus, from a toxic species of ...
Pseudo-Membrane Jackets: Two-Dimensional Coordination Polymers Achieving Visible Phase Separation in Cell Membrane KU Leuven
Cell membranes contain lateral systems that consist of various lipid compositions and actin cytoskeleton, providing two-dimensional (2D) platforms for chemical reactions. However, such complex 2D environments have not yet been used as a synthetic platform for artificial 2D nanomaterials. Herein, we demonstrate the direct synthesis of 2D coordination polymers (CPs) at the liquid-cell interface of the plasma membrane of living cells. The ...
Poliovirus separation from cell extracts using capillary electrophoresis: potential use in vaccine production and control ? Vrije Universiteit Brussel
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Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq KU Leuven
Parallel sequencing of a single cell's genome and transcriptome provides a powerful tool for dissecting genetic variation and its relationship with gene expression. Here we present a detailed protocol for G&T-seq, a method for separation and parallel sequencing of genomic DNA and full-length polyA(+) mRNA from single cells. We provide step-by-step instructions for the isolation and lysis of single cells; the physical separation of polyA(+) ...
Vector separation of particles and cells using an array of slanted open cavities KU Leuven
We present a microfluidic platform for the continuous separation of suspended particles based on their size and settling velocity. The separation method takes advantage of the flow field in the vicinity and inside slanted open cavities. These cavities induce flow along them, which deflects the suspended particles to a different degree depending on the extent to which they penetrate into the cavities. The cumulative deflection in the periodic ...
Enrichment of persister cells through Β-lactam-induced filamentation and size separation KU Leuven
Analyzing persisters at the single-cell level is crucial to properly define their phenotypic traits. However, single-cell analyses are challenging due to the rare and temporary nature of persister cells, thus requiring their rapid and efficient enrichment in a culture. Existing methods to isolate persisters from a bacterial population show important shortcomings, including contamination with susceptible cells and/or cell debris, which complicate ...
Selective separation of microalgae cells using inertial microfluidics KU Leuven
Microalgae represent the most promising new source of biomass for the world's growing demands. However, the biomass productivity and quality is significantly decreased by the presence of bacteria or other invading microalgae species in the cultures. We therefore report a low-cost spiral-microchannel that can effectively separate and purify Tetraselmis suecica (lipid-rich microalgae) cultures from Phaeodactylum tricornutum (invasive diatom). ...