Publicaties
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Biological Assay to Determine Gonadotropin Potency: From In Vivo to In Vitro Sustainable Method KU Leuven
Various preparations of follicle-stimulating hormone (FSH) are commercially available; however, they differ in glycoforms composition and purity owing to their respective sources. Additional chemical/physical changes can also be introduced during manufacturing and can impact their biological activity (biopotency), which is routinely assessed using an in vivo bioassay (Steelman-Pohley). This study aimed to determine whether an in vitro bioassay ...
Antiprotozoan lead discovery by aligning dry and wet screening: Prediction, synthesis, and biological assay of novel quinoxalinones KU Leuven
Protozoan parasites have been one of the most significant public health problems for centuries and several human infections caused by them have massive global impact. Most of the current drugs used to treat these illnesses have been used for decades and have many limitations such as the emergence of drug resistance, severe side-effects, low-to-medium drug efficacy, administration routes, cost, etc. These drugs have been largely neglected as ...
Synthesis and biological evaluation of small-molecule fusion compounds for the optimization of MASPIT, a three-hybrid target deconvolution assay Universiteit Gent
Design, synthesis and biological evaluation of tamoxifen-fusion compounds for the optimization of MASPIT, a three-hybrid target deconvolution assay Universiteit Gent
Synthesis and biological evaluation of tamoxifen fusion compounds for the optimization of MASPIT, a three-hybrid target deconvolution assay Universiteit Gent
Activity-based reporter assays for the screening of abused substances in biological matrices Universiteit Gent
Repurposing High-Throughput Image Assays Enables Biological Activity Prediction for Drug Discovery KU Leuven
In both academia and the pharmaceutical industry, large-scale assays for drug discovery are expensive and often impractical, particularly for the increasingly important physiologically relevant model systems that require primary cells, organoids, whole organisms, or expensive or rare reagents. We hypothesized that data from a single high-throughput imaging assay can be repurposed to predict the biological activity of compounds in other assays, ...
Method comparison of dipeptidyl peptidase IV activity assays and their application in biological samples containing reversible inhibitors Universiteit Antwerpen
Background Dipeptidyl peptidase IV (DPPIV, DPP4) is a serine protease that releases N-terminal dipeptides. It is a validated drug target for type 2 diabetes and DPPIV inhibitors are currently evaluated for other therapeutic applications. Various assays are used for DPPIV activity measurements in biological samples. Highly sensitive methods are needed to measure also very low activities in inhibited samples. Methods Here, the three most ...
Correlated quantification using microbiological and electrochemical assays for roxithromycin determination in biological and pharmaceutical samples KU Leuven
Microbiological and electrochemical assays, applying the cylinder-plate and differential pulse voltammetry as techniques, are reported for the quantitative determination of roxithromycin in serum and solid pharmaceutical form. The microbiological assay is based upon the inhibitory effect of this drug on the strain Bacillus subtilis ATCC 9372 used as the test microorganism. Linearity of the calibration curve was observed over the concentration ...