The y-secretase substrate proteome and its role in cell signaling regulation Universiteit Antwerpen Vlaams Instituut voor Biotechnologie KU Leuven
y-Secretases mediate the regulated intramembrane proteolysis (RIP) of more than 150 integral membrane proteins. We developed an unbiased y-secretase substrate identification (G-SECSI) method to study to what extent these proteins are processed in parallel. We demonstrate here parallel processing of at least 85 membrane proteins in human microglia in steady-state cell culture conditions. Pharmacological inhibition of y-secretase caused ...