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DNA metabarcoding on repeat: Sequencing data of marine macrobenthos are reproducible and robust across labs and protocols Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
DNA metabarcoding can be used in marine environmental monitoring if results are reproducible between labs and robust against modifications to the lab protocol. In this interlaboratory study, we conducted a ring test where subsamples of blended macrobenthos samples were distributed to four laboratories located in Belgium, the Netherlands, Germany and Denmark. Samples were processed by a standardized lab protocol and by an adapted protocol, and ...
DNA-based monitoring of soft sediments Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
DNA-based monitoring has the potential to provide high resolution biodiversity data from the marine environment at a fraction of the time and costs associated with morphology based monitoring. Yet, proper ground truthing of DNA-based monitoring is needed across different member states to reach harmonization of methods and to pave the way for implementation in national and European monitoring programs. Within the GEANS soft sediment pilot, three ...
Evaluation of DNA metabarcoding using Oxford Nanopore sequencing for authentication of mixed seafood products Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Seafood is the world's most highly traded food commodity. Due to the non-stop demand of the ever-increasing human population, the supply of certain species is not guaranteed, and mislabelling practices arise. Fillets and heavily processed products that cannot be easily authenticated are particularly vulnerable to fraudulent practices. In order to face this challenge, as a cost-effective, rapid, and easy handling tool, the MinION sequencer from ...
Valorisation of crustacean and bivalve processing side streams for industrial fast time-to-market products: A review from the European Union regulation perspective Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent
A massive amount of crustaceans and bivalves are consumed each year, leading to millions of tons of processing side streams from the seafood industry. Considering the current trend of (bio)circular and zero-waste food production, crustacean and bivalve processing side streams (CBPS) seem a promising and emerging resource for producing high-value-added products. This paper highlights the general composition of CBPS with high commercial values, ...
Microbiome Differentiation Among Coexisting Nematode Species in Estuarine Microhabitats: A Metagenetic Analysis Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent
Resource partitioning is a mechanism for niche differentiation which can facilitate coexistence of species at local scales. Insights into resource use and niche differentiation of functionally similar and/or taxonomically closely related species are scant, especially so for small organisms that dominate marine sediments in terms of abundance and species richness. In this study we characterized the microbiomes of 10 bacterivorous nematode species ...
The initial response of females towards congeneric males matches the propensity to hybridise in Ophthalmotilapia Instituut voor Landbouw-, Visserij- en Voedingsonderzoek KU Leuven Universiteit Antwerpen
Cichlid radiations often harbour closely related species with overlapping niches and distribution ranges. Such species sometimes hybridise in nature, which raises the question how they can coexist. This also holds for the Tanganyika mouthbrooders Ophthalmotilapia ventralis and O. nasuta. Earlier studies found indications of asymmetrical hybridisation with females of O. ventralis accepting males of O. nasuta, but not the other way around. We ...
Te weinig data over tong in 7hjk: Nieuw onderzoek tracht via (e)DNA de gaten te vullen Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Vissers die zich richten op het tongbestand in de zuidelijke Keltische Zee en het zuidwesten van Ierland (ICES-gebieden 7hjk) worden de laatste jaren geconfronteerd met weinig positieve adviezen en dalende TACs. De oorzaak daarvan is een tekort aan data en de daaruit vloeiende onduidelijkheid over de bestandsgrootte (biomassa) en de genetische identiteit van de stock. Het nieuwe EFMZV project SoleDNA, uitgevoerd door ILVO zal genetische ...
Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR) Instituut voor Landbouw-, Visserij- en Voedingsonderzoek KU Leuven
Fishery products are often subject to substitution fraud, which is hard to trace due to a lack of morphologic traits when processed, gutted, or decapitated. Traditional molecular methods (DNA barcoding) fail to identify products containing multiple species and cannot estimate original weight percentages. As a proof of concept, an Atlantic salmon (Salmo salar) specific ddPCR assay was designed to authenticate mixed food products. The method ...
Application of tools to identify seafood substitution Instituut voor Landbouw-, Visserij- en Voedingsonderzoek
Seafood is an important source of protein for humans but is prone to substitution fraud. Substitution fraud comprises the replacement of an expensive species with a cheaper alternative, with the intent to make a financial gain. Earlier this year, The Guardian reported that, globally, 36 % of seafood samples were mislabelled. Substitution of seafood can have economical, ecological, ethical, and health consequences, as well as serve as a cover for ...