Publicaties
Use of hyperglycemic clamp to assess pancreatectomy and islet cell autotransplant in patient with heterotaxy syndrome and dorsal pancreas agenesis leading to chronic pancreatitis Vrije Universiteit Brussel
Patients with heterotaxy syndrome (HS) can present with an associated complete dorsal pancreas agenesis (DPA). They are considered to be at increased risk for developing diabetes due to a reduced functional beta cell mass (FBM) as well as for chronic pancreatitis leading to unmanageable pain. We report the case of a young female with chronic pancreatitis due to HS and associated DPA. She presented with a severe persisting upper abdominal pain ...
Insulitis and lymphoid structures in the islets of Langerhans of a 66-year-old patient with long-standing type 1 diabetes Vrije Universiteit Brussel
Insulitis is a characteristic inflammatory lesion consisting of immune cell infiltrates around and within the pancreatic islets of patients with recent-onset type 1 diabetes (T1D). The infiltration is typically mild, both in terms of the number of infiltrating cells and the number of islets affected. Here, we present an unusual histopathological case study of a 66-year-old female patient with long-standing T1D, insulitis, and islet-associated ...
Evidence of tissue repair in human donor pancreas after prolonged duration of stay in intensive care. Vrije Universiteit Brussel
M2 macrophages play an important role in tissue repair and regeneration. They have also been found to modulate β-cell replication in mouse models of pancreatic injury and disease. We previously reported that β-cell replication is strongly increased in a subgroup of human organ donors characterized by prolonged duration of stay in an intensive care unit (ICU) and increased number of leukocytes in the pancreatic tissue. In the present study we ...
Heterogeneity of Human Pancreatic Islet Isolation Around Europe UZBrussel
Background. Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading
groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization
of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation
processes within European countries. Methods. A ...
groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization
of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation
processes within European countries. Methods. A ...
Use of culture to reach metabolically adequate beta cell dose by combining donor islet cell isolates for transplantation in type 1 diabetes patients Vrije Universiteit Brussel
BACKGROUND: Clinical islet transplantation is generally conducted within 72 hours after isolating sufficient beta cell mass. A preparation that does not meet the sufficient dose can be cultured until this is reached after combination with subsequent ones. This retrospective study examines whether metabolic outcome is influenced by culture duration.
METHOD: Forty type 1 diabetes recipients of intraportal islet cell grafts under ATG ...
Cell Mass Increase Associated with Formation of Glucose-Controlling beta-Cell Mass in Device-Encapsulated Implants of hiPS-Derived Pancreatic Endoderm Vrije Universiteit Brussel
Device-encapsulated human stem cell-derived pancreatic endoderm (PE) can generate functional β-cell implants in the subcutis of mice, which has led to the start of clinical studies in type 1 diabetes. Assessment of the formed functional β-cell mass (FBM) and its correlation with in vivo metabolic markers can guide clinical translation. We recently reported ex vivo characteristics of device-encapsulated human embryonic stem cell-derived ...
Bètaceltransplantatie: indicaties en doelstellingen anno 2019 Vrije Universiteit Brussel
Bètacelvervangende therapie is een behandelingsoptie bij diabetespatiënten met onderliggend bètacelfalen. Welke patiënten komen hiervoor in aanmerking en wat zijn de te verwachten voordelen van dergelijke therapie? Hierover werd recent een consensusrapport gepubliceerd door een werkgroep samengesteld uit opinieleiders van zowel de International als de European Pancreas and Islet Transplantation Association (IPITA/EPITA). Een korte samenvatting ...
A composite immune signature parallels disease progression across T1D subjects UZBrussel
At diagnosis, most people with type 1 diabetes (T1D) produce measurable levels of endogenous insulin, but the rate at which insulin secretion declines is heterogeneous. To explain this heterogeneity, we sought to identify a composite signature predictive of insulin secretion, using a collaborative assay evaluation and analysis pipeline that incorporated multiple cellular and serum measures reflecting β cell health and immune system activity. ...
Immune checkpoint inhibitors and type 1 diabetes mellitus UZBrussel
Objective: To better define the rare adverse event (AE) of diabetes mellitus associated with immune checkpoint inhibitors (ICIs). Design and methods: We report the case of a lung cancer patient with diabetic ketoacidosis (DKA) and autoimmune thyroiditis during pembrolizumab treatment. We provide a systematic review of all published cases (PubMed/Web of Science/Cochrane, through November 2018) of autoimmune diabetes mellitus related to ...