Publicaties

Layer-by-Layer coating technology is of great importance for many applications of microparticles whereby exposure of the particles to various reagents is needed. Mutual contamination of the reagents during this process is a key challenge, and this undesired effect should be avoided. Here we introduce a device that provides subsequent exposure of particles to various liquids and minimizes mixing of the liquids at the same time. The key element ...

Impurities control the formation of bio-crystals and can fully paralyze crystal growth at low levels of supersaturation. Traditional impurity models predict that an escape from this so-called “dead zone” requires an increase in the driving force (i.e. supersaturation). In this work, using protein crystals as a model system, we uncover an alternative escape route from the dead zone that does not involve an increase in supersaturation. We ...

A new kinetic Monte Carlo simulation approach for crystal growth from solution is presented. The simulations include an explicit representation of an extended volume of solution above the crystal face and realistically treats voids within the forming crystal, step overhangs and concentration gradients in the mother solution. The effect of impurities on step growth is investigated and it is shown that the results depend on a complex interplay ...

Protein self-assembly into fibrils and oligomers plays a key role in the etiology of degenerative diseases. Several pathways for this self-assembly process have been described and shown to result in different types and ratios of final assemblies, therewith defining the effective physiological response. Known factors that influence assembly pathways are chemical conditions and the presence or lack of agitation. However, in natural and ...

This paper presents a rail guided method to apply a Layer-by-Layer (LbL) coating on particles in a microfluidic device. The passive microfluidic approach allows handling suspensions of particles to be coated in the system. The trajectory of the particles is controlled using engraved rails, inducing lateral movement of particles while keeping the axially oriented liquid flow (and the interface of different liquids) undisturbed. The depth and ...

We report the investigation of various experimental conditions and their influence on pol-ymorphism of 5-methyl-2-[(2-nitrophenyl)amino]-3-thiophenecarbonitrile, commonly known as ROY. These conditions include an in-house-developed microfluidic chip with controlled mixing of parallel flows. We observed that different ROY concentrations and different solvent to antisolvent ratios naturally favored different polymorphs. Nonetheless, identical ...

Induced fit- (IF) and conformational selection (CS) binding mechanisms have long been regarded to be mutually exclusive. Yet, they are now increasingly considered to produce the final ligand-target complex alongside within a thermodynamic cycle. This viewpoint benefited from the introduction of binding fluxes as a tool for analyzing the overall behavior of such cycle. This study aims to provide more vivid and applicable insights into this ...