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Sera from dams of calves with bovine neonatal pancytopenia contain alloimmune antibodies directed against calf leukocytes KU Leuven
Bovine neonatal pancytopenia (BNP) is a bleeding and pancytopenic syndrome in neonatal calves, which recently emerged all over Europe. The present study tested whether antibodies directed against calf leukocytes are present in sera from known BNP dams. Sera from BNP dams (n=11) were combined with leukocytes from 11 calves (5 BNP survivors and 6 controls). After adding a fluorescein conjugated F(ab')(2) fragment of rabbit anti-bovine IgG ...
OXALIPLATIN ELICITS MECHANICAL AND COLD ALLODYNIA IN RODENTS VIA TRPA1 RECEPTOR STIMULATION KU Leuven
Serum amyloid A3 (SAA3), not SAA1 appears to be the major acute phase SAA isoform in the pig KU Leuven
The acute-phase serum amyloid A (SAA) protein family comprises two main circulating (systemic) isoforms, SAA1 and SAA2, synthesised in liver and one local isoform, SAA3, produced in extrahepatic tissues. Systemic and local SAA show structural differences, which suggests different functions. In the pig, AA-amyloidosis is extremely uncommon, and the structural protein in swine has characteristics of systemic SAA. The only pig SAA sequences ...
Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding KU Leuven
Enterohaemorrhagic Escherichia coli O157:H7 are zoonotic pathogens associated with haemorrhagic colitis (HC) and the haemolytic uremic syndrome (HUS). Ruminants are the main reservoir of this organism and most outbreaks of E. coli O157:H7 infections are food borne. Food contamination by ruminant manure has been reported as the primary source of human infection, therefore inhibition of E. coli O157:H7 colonization and shedding in ruminants could ...
Oral administration of beta-1,3/1,6-glucan Macrogard fails to enhance the mucosal immune response following oral F4 fimbrial immunisation in gnotobiotic pigs KU Leuven
In this study gnotobiotic animals were used to see if the F4 fimbrial antigen of F4ac+ Escherichia coli is as immunogenic as in conventional pigs. In addition, the adjuvant effect of beta-1,3/1,6-glucans was analysed for the first time in germ-free domestic animals. Oral F4 immunisation of F4 receptor positive (F4R(+)) piglets was able to induce a systemic and mucosal immune response as conventional pigs, although less pronounced. Nevertheless, ...
Clathrin-mediated endocytosis and transcytosis of enterotoxigenic Escherichia coli F4 fimbriae in porcine intestinal epithelial cells KU Leuven
Enterotoxigenic Escherichia coli (ETEC) cause severe diarrhea in neonatal and recently weaned piglets. Previously, we demonstrated that oral immunization of F4 receptor positive piglets with purified F4 fimbriae induces a protective F4-specific intestinal immune response. However, in F4 receptor negative animals no F4-specific immune response can be elicited, indicating that the induction of an F4-specific mucosal immune response upon oral ...