Publicaties

1 - 10 of 910 resultaten

Zoek term:

Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case KU Leuven

Sara Abouelasrar Salama, Mirre De Bondt, Nele Berghmans, Mieke Gouwy, Vivian Louise Soares de Oliveira, Sergio C Oliveira, Flavio A Amaral, Paul Proost, Jozef Van Damme, Sofie Struyf, et al.
The serum amyloid A (SAA) gene family is highly conserved and encodes acute phase proteins that are upregulated in response to inflammatory triggers. Over the years, a considerable amount of literature has been published attributing a wide range of biological effects to SAAs such as leukocyte recruitment, cytokine and chemokine expression and induction of matrix metalloproteinases. Furthermore, SAAs have also been linked to protumorigenic, ...

Live Cell Imaging Demonstrates Multiple Routes Toward a STAT1 Gain-of-Function Phenotype KU Leuven

Simone Giovannozzi, Veerle Lemmens, Jelle Hendrix, Rik Gijsbers, Rik Schrijvers
Signal transducer and activator of transcription 1 (STAT1) gain-of-function (GOF) mutations result in a primary immunodeficiency (PID) characterized typically by chronic mucocutaneous candidiasis (CMC), but a wider phenotypic range is reported and remains unexplained from a pathophysiological point-of-view. We hypothesized that different STAT1 GOF mutations may result in distinct molecular mechanisms, possibly explaining the variable phenotypes ...

Intestinal Delivery of Proinsulin and IL-10 viaLactococcus lactisCombined With Low-Dose Anti-CD3 Restores Tolerance Outside the Window of Acute Type 1 Diabetes Diagnosis KU Leuven

Dana P Cook, Joao Paulo Monteiro Carvalho Mori Cunha, Pieter-Jan Martens, Gabriele Sassi, Francesca Mancarella, Giuliana Ventriglia, Guido Sebastiani, An-Sofie Vanherwegen, Mark A Atkinson, Karolien Van Huynegem, et al.
A combination treatment (CT) of proinsulin and IL-10 orally delivered via genetically modified Lactococcus lactis bacteria combined with low-dose anti-CD3 (aCD3) therapy successfully restores glucose homeostasis in newly diagnosed non-obese diabetic (NOD) mice. Tolerance is accompanied by the accumulation of Foxp3+ regulatory T cells (Tregs) in the pancreas. To test the potential of this therapy outside the window of acute diabetes diagnosis, we ...

A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute ExperimentalTrypanosoma bruceiInfection KU Leuven Universiteit Gent Vrije Universiteit Brussel

Carl De Trez, Benoit Stijlemans, Viki Bockstal, Jennifer Cnops, Johanna Korf, Jacques Van Snick, Guy Caljon, Eric Muraille, Ian R Humphreys, Louis Boon, et al.
In many infectious diseases, the immune response operates as a double-edged sword. While required for protective immunity, infection-induced inflammation can be detrimental if it is not properly controlled, causing collateral body damage and potentially leading to death. It is in this context that the potent anti-inflammatory cytokine interleukin-10 (IL-10) is required to dampen the pro-inflammatory immune response that hallmarks trypanosomosis. ...

A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection. Vrije Universiteit Brussel KU Leuven Universiteit Gent

Carl De Trez, Benoit Stijlemans, Viki Bockstal, Jennifer Cnops, Korf Hannelies, Jacques Van Snick, Guy Caljon, Eric Muraille, Ian R Humphreys, Louis Boon, et al.

In many infectious diseases, the immune response operates as a double-edged sword. While required for protective immunity, infection-induced inflammation can be detrimental if it is not properly controlled, causing collateral body damage and potentially leading to death. It is in this context that the potent anti-inflammatory cytokine interleukin-10 (IL-10) is required to dampen the pro-inflammatory immune response that hallmarks ...

Truncation of CXCL8 to CXCL8(9-77) enhances actin polymerization and in vivo migration of neutrophils KU Leuven

Maria Dorothée Metzemaekers, Sofie Vandendriessche, Nele Berghmans, Mieke Gouwy, Paul Proost
CXCL8 is the principal human neutrophil-attracting chemokine and a major mediator of inflammation. The chemokine exerts its neutrophil-chemotactic and neutrophil-activating activities via interaction with glycosaminoglycans (GAGs) and activation of the G protein-coupled receptors (GPCRs) CXCR1 and CXCR2. Natural CXCL8 displays an exceptional degree of amino (NH2 )-terminal heterogeneity. Most CXCL8 forms result from proteolytic processing of ...

Serum Amyloid A1 (SAA1) Revisited: Restricted Leukocyte-Activating Properties of Homogeneous SAA1 KU Leuven

Sara Abouelasrar Salama, Mirre De Bondt, Mieke De Buck, Nele Berghmans, Paul Proost, Vivian Louise Soares Oliveira, Flavio A Amaral, Mieke Gouwy, Jozef Van Damme, Sofie Struyf, et al.
Infection, sterile injury, and chronic inflammation trigger the acute phase response in order to re-establish homeostasis. This response includes production of positive acute phase proteins in the liver, such as members of the serum amyloid A (SAA) family. In humans the major acute phase SAAs comprise a group of closely related variants of SAA1 and SAA2. SAA1 was proven to be chemotactic for several leukocyte subtypes through activation of the G ...

TRPV4 Mediates Acute Bladder Responses to Bacterial Lipopolysaccharides KU Leuven

Yeranddy A Alpizar, Pieter Uvin, Robbe Naert, Jan Franken, Silvia Pinto, Alicia Sanchez, Thomas Gevaert, Wouter Everaerts, Thomas Voets, Dirk De Ridder, et al.
Urinary tract infections (UTI) affect a large proportion of the population, causing among other symptoms, more frequent and urgent micturition. Previous studies reported that the gram-negative bacterial wall component lipopolysaccharides (LPS) trigger acute epithelial and bladder voiding responses, but the underlying mechanisms remain unknown. The cation channel TRPV4 is implicated in the regulation of the bladder voiding. Since TRPV4 is ...