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Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Tijdschriftbijdrage - Tijdschriftartikel

Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

Tijdschrift: Tuberculosis
ISSN: 1472-9792
Volume: 100
Pagina's: 89-94
Jaar van publicatie:2016
Trefwoorden:Mycobacterium tuberculosis/genetics, Mycobacterium tuberculosis/physiology, Sputum/microbiology, Bronchoalveolar lavage/microbiology, Computational biology/methods, * Gene expression profiling, Tuberculosis, pulmonary/epidemiology/*microbiology, GENE-EXPRESSION, ADAPTATION, PERSISTENCE