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Possible influence of surfactants and proteins on the efficiency of contact agar microbiological surface sampling
Tijdschriftbijdrage - Tijdschriftartikel
Agar contact microbiological sampling techniques, based on a transfer of the microorganisms present on a surface to a
culture medium, are widely used to assess and control surface cleanliness and to evaluate microbial contamination levels. The
effectiveness of these techniques depends on many environmental parameters that influence the strength of attachment of the
bacteria to the surface. In the present study, stainless steel and high density polyethylene surfaces were inoculated with known
concentrations of Staphylococcus epidermidis. Following an experimental design, the surfaces were sampled with different types
of replicate organism direct agar contact plates and Petrifilm; results indicated that recovery rates were influenced by the presence
of egg white albumin or Tween 80 in the inoculum solutions or by the introduction of surfactants into the contact agar of the
microbiological sampling techniques. The techniques yielded significantly different results, depending on sampling conditions,
underlining the need for a standardization of laboratory experiments to allow relevant comparisons of such techniques.
Tijdschrift: Journal of Food Protection
ISSN: 0362-028X
Issue: 11
Volume: 73
Pagina's: 2116 - 2122
Jaar van publicatie:2010
BOF-keylabel:ja
IOF-keylabel:ja
BOF-publication weight:1
CSS-citation score:1
Authors from:Higher Education