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Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Tijdschriftbijdrage - Tijdschriftartikel

Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.
Tijdschrift: Nature Methods
ISSN: 1548-7091
Issue: 8
Volume: 16
Pagina's: 707 - +
Aantal pagina's: 7
Jaar van publicatie:2019