< Terug naar vorige pagina


Independent movement of the voltage sensors in K(V)2.1/K(V)6.4 heterotetramers

Tijdschriftbijdrage - e-publicatie

Heterotetramer voltage-gated K+ (K-V) channels K(V)2.1/K(V)6.4 display a gating charge-voltage (Q(V)) distribution composed by two separate components. We use state dependent chemical accessibility to cysteines substituted in either K(V)2.1 or K(V)6.4 to assess the voltage sensor movements of each subunit. By comparing the voltage dependences of chemical modification and gating charge displacement, here we show that each gating charge component corresponds to a specific subunit forming the heterotetramer. The voltage sensors from K(V)6.4 subunits move at more negative potentials than the voltage sensors belonging to K(V)2.1 subunits. These results indicate that the voltage sensors from the tetrameric channels move independently. In addition, our data shows that 75% of the total charge is attributed to K(V)2.1, while 25% to K(V)6.4. Thus, the most parsimonious model for K(V)2.1/K(V)6.4 channels' stoichiometry is 3:1.
Tijdschrift: Scientific reports
ISSN: 2045-2322
Volume: 7
Jaar van publicatie:2017
Trefwoorden:A1 Journal article
BOF-publication weight:3
CSS-citation score:1
Authors from:Higher Education