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High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation
Tijdschriftbijdrage - Tijdschriftartikel
To protect allergic patients and guarantee correct food labeling, robust, specific and sensitive detection methods are urgently needed. Mass spectrometry (MS)-based methods could overcome the limitations of current detection techniques. The first step in the development of an MS-based method is the identification of biomarkers, which are, in the case of food allergens, peptides. Here, we implemented a strategy to identify the most salient peptide biomarkers in peanuts. Processed peanut matrices were prepared and analyzed using an untargeted approach via high-resolution MS. More than 300 identified peptides were further filtered using selection criteria to strengthen the analytical performance of a future, routine quantitative method. The resulting 16 peptides are robust to food processing, specific to peanuts, and satisfy sequence-based criteria. The aspect of multiple protein isoforms is also considered in the selection tree, an aspect that is essential for a quantitative method's robustness but seldom, if ever, considered.
Tijdschrift: Food Chemistry
Jaar van publicatie:2020
Trefwoorden:High-resolution mass spectrometry, Multiple allergen isoforms, Peanut origin, Peptide biomarker selection, Processed food products