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Endocrine activity in waste and river waters from the Brussels Region, Belgium using the BG1Luc4E2 CALUX Bioassay - Abstract @ Dioxin 2016
Boekbijdrage - Boekhoofdstuk Conferentiebijdrage
Today, there is international concern regarding the effects of natural and synthetic chemicals on the health of humans and wildlife since these emerging pollutants are able to interfere and act upon the hormonal system. These so-called endocrine-disrupting chemicals (EDCs) or endocrine active chemicals (EACs) are of particular concern to aquatic ecosystems, because these compounds are present in almost all wastewater and treated wastewater effluents and in rivers receiving these effluents, ground water supplies, sea water, sediment and biota, and could be of major concern for urban river systems such as the Zenne River in Brussels, Belgium1,2.
In this respect, the European Water Framework Directive (WFD)3, whose main objective was to obtain a good ecological and chemical status for all European water bodies by 2015, established a priority list of 33 new and 8 previously regulated chemical pollutants presenting a significant risk to or via the aquatic environment. For the priority substances, environmental quality standards were set in 2008, and they have to be monitored by all EU-Member States3. Several of these substances are recognized endocrine disrupting chemicals such as the penta-bromodiphenylether (PBDE), octylphenol (OP), nonylphenol (NP) and the di(2-ethylhexyl)phthalate (DEHP), and are listed as hazardous priority substances4. In addition, several (natural) hormones have been included in the WFD Watch List3, among them 17β-estradiol (E2) and ethinylestradiol (EE2).
Rather than looking at individual concentrations of these compounds using hyphenated chromatography techniques coupled to mass detection, biologically relevant and integrative approaches exist to perform a first screening basis for endocrine activity5,6. For this reason, an in vitro trans-activational reporter gene assay, BG1Luc4E2 cell line7,8, was employed to carry out endocrine activity determination in both Zenne River water, WWTP (waste water treatment plant) influents and effluents and also hospital effluent from the Brussels Region.
In this respect, the European Water Framework Directive (WFD)3, whose main objective was to obtain a good ecological and chemical status for all European water bodies by 2015, established a priority list of 33 new and 8 previously regulated chemical pollutants presenting a significant risk to or via the aquatic environment. For the priority substances, environmental quality standards were set in 2008, and they have to be monitored by all EU-Member States3. Several of these substances are recognized endocrine disrupting chemicals such as the penta-bromodiphenylether (PBDE), octylphenol (OP), nonylphenol (NP) and the di(2-ethylhexyl)phthalate (DEHP), and are listed as hazardous priority substances4. In addition, several (natural) hormones have been included in the WFD Watch List3, among them 17β-estradiol (E2) and ethinylestradiol (EE2).
Rather than looking at individual concentrations of these compounds using hyphenated chromatography techniques coupled to mass detection, biologically relevant and integrative approaches exist to perform a first screening basis for endocrine activity5,6. For this reason, an in vitro trans-activational reporter gene assay, BG1Luc4E2 cell line7,8, was employed to carry out endocrine activity determination in both Zenne River water, WWTP (waste water treatment plant) influents and effluents and also hospital effluent from the Brussels Region.
Boek: Dioxin 2016, 36th International Symposium on Halogenated Persistent Organic Pollutants
Volume: 78
Pagina's: 785-788
Aantal pagina's: 4
Jaar van publicatie:2017
Trefwoorden:CALUX, Dioxin, Optimization
Toegankelijkheid:Open