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Project
Karakterisatie van signaaltransductiewegen die betrokken zijn bij de differentiatie van pluripotente stamcellen tot levercellen.
Embryonic stem cells can undergo extensive self-renewing cell divisionsand differentiate into all cell types. </>The Verfaillie lab developed a protocol to differentiate pluripotent stem cells towards mature hepatocyte-like cells. The protocol uses growth factor cocktails that play a role in liver embryonic development, and directs the cells stepwisethrough primitive streak, mesendoderm, definitive endoderm, and finallyto hepatocyte-like cells. Tgf members play an important role indirecting this differentiation. The purpose of this project is to studythe mechanisms underlying the ability of Tgf family members to fate pluripotent cells towards endoderm. For this study we will knock down different molecules of the Tgf signaling pathway, one by one using esiRNAs, and assessing their effect on endoderm formation and their effect on the other Tgf family members .
With this study we hope gain insights into the molecular basis that is underlying certaincell fate decisions and hereby contribute in enhancing the current differentiation protocol towards hepatocyte-like cells.
With this study we hope gain insights into the molecular basis that is underlying certaincell fate decisions and hereby contribute in enhancing the current differentiation protocol towards hepatocyte-like cells.
Datum:1 aug 2010 → 31 jul 2014
Trefwoorden:Pluripotent, Embryonic, Hepatocyte-like.
Project type:PhD project