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Project
Epidemiology of brucellosis in human, domestic ruminants and swine of Bangladesh
Brucellosis, worldwide the most important zoonosis, remains a major health problem worldwide. In Bangladesh, there are sporadic reports of prevalence (cattle, goats, buffalo and man). Detailed epidemiological studies are essential to design an appropriate control strategy for the disease. The objectives of the proposed project are to estimate true prevalence, distribution, risk factors, economic impact of the disease and characterization of BrucelIa spp. infecting domestic ruminants, swine and human. Nation-wide cross-sectional surveys will be undertaken following a stratified (division) multistage (production system, age, breed, sex and season) sampling strategy. Geo-referenced bovine, ovine, caprine, porcine and bubaline milk and blood samples as well as relevant samples of farmers, veterinarians, butchers and animal health workers will be collected from 6 divisions of Bangladesh. Blood samples of patients having pyrexia of unknown origin (PUO) will be collected from hospitals. Questionnaire-based
data about hypothesized risk factors will also be collected. Multiple tests such as Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), Complement Fixation Test (Cl-T), Indirect Enzyme Linked Immunosorbent
Assay (ELISA) and Polymerase Chain Reaction (PCR) will be applied to diagnose the disease. True prevalence of brucellosis in different species will
be estimated using a Bayesian approach. The distribution of brucellosis in different species of animals and man will be determined using GIS software and spatial analysis. The GIS data generated by the project will form the framework of a brucellosis database in Bangladesh that can be updated as new information about the disease becomes available. Disease risk factors in different species will be identified using both parametric and non-parametric statistics. Inter-species transmission dynamics will be modelled by means of hierarchical Monte Carlo simulations. The economic impact of the disease both in man and animals will be estimated quantitatively. Isolation and molecular characterization of Brucella spp. will be carried out milk, blood and aborted foetal samples. Distribution of biotypes in animals and man will also be reported. The brucellosis epidemiological understanding in Bangladesh will help to design an adapted control programme.
data about hypothesized risk factors will also be collected. Multiple tests such as Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), Complement Fixation Test (Cl-T), Indirect Enzyme Linked Immunosorbent
Assay (ELISA) and Polymerase Chain Reaction (PCR) will be applied to diagnose the disease. True prevalence of brucellosis in different species will
be estimated using a Bayesian approach. The distribution of brucellosis in different species of animals and man will be determined using GIS software and spatial analysis. The GIS data generated by the project will form the framework of a brucellosis database in Bangladesh that can be updated as new information about the disease becomes available. Disease risk factors in different species will be identified using both parametric and non-parametric statistics. Inter-species transmission dynamics will be modelled by means of hierarchical Monte Carlo simulations. The economic impact of the disease both in man and animals will be estimated quantitatively. Isolation and molecular characterization of Brucella spp. will be carried out milk, blood and aborted foetal samples. Distribution of biotypes in animals and man will also be reported. The brucellosis epidemiological understanding in Bangladesh will help to design an adapted control programme.
Datum:1 mrt 2007 → 2 mrt 2015
Trefwoorden:B780-tropische-geneeskunde