Single molecule reader for identification of biopolymers

The invention relates to methods for the identification of a biopolymer comprising the steps of: (a) measuring the linear pattern of sequence specific markers on a biopolymer, (b) calculating the similarity score between the measured linear pattern and an expected linear pattern for each individual member of a user- defined set of potential target biopolymers, (c) calculating the significance for each of the obtained similarity scores, thereby obtaining a set of target biopolymers with significant similarity of the expected linear pattern to the measured linear pattern, (d) modifying the expected linear pattern of the set of target biopolymers of step c) by reshuffling, adding or removing marker positions from the expected pattern of said target biopolymers, (e) calculating the similarity score between the measured linear pattern and the modified expected linear pattern of step d) for each individual member of the significant set of potential target biopolymers of step b), and (f) calculating the significance for each of the obtained similarity scores, thereby obtaining, compared to the set of target biopolymers of step c, a reduced set of target biopolymers with significant similarity, resulting in improved specificity without loss of sensitivity for the identification of the measured sequence.

Octrooi-publicatienummer: WO2020089337

Jaar aanvraag: 2020

Jaar toekenning: 2021

Jaar van publicatie: 2020

Status: Aangevraagd

Technologiedomeinen: Analyse van biologische materialen, Biotechnologie

Gevalideerd voor IOF-sleutel: Ja

Toegewezen aan: Associatie KULeuven