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Culturomics to investigate the Endometrial Microbiome: Proof-of-Concept

Tijdschriftbijdrage - Tijdschriftartikel

Ondertitel:Proof-of-Concept
Abstract: The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endome-trial microbiome is of particular interest given the potential impact on the embryo implanta-tion. To date, all endometrial microbiome studies have applied a metagenomics approach. A se-quencing-based technique, however, has its limitations, more specifically in adequately explor-ing low-biomass settings, such as intra-uterine/endometrial samples. In this proof-of-concept study, we demonstrate the applicability of culturomics, a high-throughput culturing approach, to investigate the endometrial microbiome. Ten subfertile women undergoing diagnostic hys-teroscopy and endometrial biopsy, as part of their routine work-up at Brussels IVF, were includ-ed after their informed consent. Biopsies were used to culture microbiota for up to 30 days in multiple aerobic and anaerobic conditions. Subsequent WASPLab®-assisted culturomics enabled a standardized methodology. Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) or 16S rRNA sequencing was applied to identify all of bacterial and fungal isolates. Eighty-three bacterial and two fungal species were identified. The detected species were in concordance with previously published metagenomics-based endometrial mi-crobiota analyses as 77 (91%) of them belonged to previously described genera. Nevertheless, highlighting the added value of culturomics to identify most isolates at the species level, 53 (62.4%) of the identified species were described in the endometrial microbiota for the first time. This study shows the applicability and added value of WASPLab®-assisted culturomics to inves-tigate the low biomass endometrial microbiome at a species level.
Tijdschrift: International Journal of Molecular Sciences
ISSN: 1661-6596
Issue: 20
Volume: 23
Pagina's: 1-21
Jaar van publicatie:2022
Trefwoorden:: endometrial microbiome; culturomics; ART; embryo implantation; MALDI-TOF; 16S rRNA
  • PubMed Central Id: PMC9602868
  • WoS Id: 000873014200001
  • Institutional Repository URL: https://www.mdpi.com/1422-0067/23/20/12212
  • ORCID: /0000-0002-5589-502X/work/125070421
  • Scopus Id: 85140817394
  • PubMed Id: 36293066
  • ORCID: /0000-0001-9765-1624/work/121062669
  • ORCID: /0000-0002-4281-9749/work/121050551
  • ORCID: /0000-0002-6217-1695/work/121035952
  • ORCID: /0000-0001-7012-0436/work/121032221
  • ORCID: /0000-0002-7756-3691/work/121033055
  • ORCID: /0000-0002-6410-9246/work/121035235
  • ORCID: /0000-0001-6234-3285/work/121055058
  • DOI: https://doi.org/10.3390/ijms232012212
Toegankelijkheid:Open