Similarities in the endocrine-disrupting potencies of indoor dust and flame retardants by using human osteosarcoma (U2OS) cell-based reporter gene assays

Indoor dust is a sink for many kinds of pollutants, including flame retardants (FRs), plasticizers, and their contaminants and degradation products. These pollutants can be migrated to indoor dust from household items such as televisions and computers. To reveal high-priority end points of and contaminant candidates in indoor dust, using CALUX reporter gene assays based on human osteosarcoma (U2OS) cell lines, we evaluated and characterized the endocrine-disrupting potencies of crude extracts of indoor dust collected from Japan (n = 8), the United States (n = 21), Vietnam (n = 10), the Philippines (n = 17), and Indonesia (n = 10) and for 23 selected FRs. The CALUX reporter gene assays used were specific for compounds interacting with the human androgen receptor (AR), estrogen receptor alpha (ER alpha), progesterone receptor (PR), glucocorticoid receptor (GR), and peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2). Indoor dust extracts were agonistic to ER alpha, GR, and PPAR gamma 2 and antagonistic against AR, PR, GB., and PPAR gamma 2. In comparison, a majority of FRs was agonistic to ER alpha and PPAR gamma 2 only, and some FRs demonstrated receptor-specific antagonism against all tested nuclear receptors. Hierarchical clustering clearly indicated that agonism of ER alpha and antagonism of AR and PR were common, frequently detected end points for indoor dust and tested FRs. Given our previous results regarding the concentrations of FRs in indoor dust and in light of our current results, candidate contributors to these effects include not only internationally controlled brominated FRs but also alternatives such as some phosphorus-containing FRs. In the context of indoor pollution, high-frequency effects of FRs such as agonism of ER alpha and antagonism of AR and PR are candidate high-priority end points for further investigation.

Jaar van publicatie:2013

Trefwoorden:A1 Journal article

Toegankelijkheid:Closed