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Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy

Tijdschriftbijdrage - Tijdschriftartikel

Synthesis of an array of differentially sulfated GlcN-IdoA disaccharides, accessible on good scale, directly from L-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable L-IdoA O-2 and D-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman-ROA towards fingerprinting sulfation in heparin fragments. (C) 2014 The Authors. Published by Elsevier Ltd.
Tijdschrift: Carbohydrate research
ISSN: 0008-6215
Volume: 400
Pagina's: 44 - 53
Jaar van publicatie:2014
Trefwoorden:A1 Journal article
BOF-keylabel:ja
BOF-publication weight:1
CSS-citation score:1
Auteurs:International
Authors from:Higher Education
Toegankelijkheid:Closed