Intratracheal Budesonide/Surfactant attenuates hyperoxia-induced lung injury in preterm rabbits.

Recent clinical trials have shown improvements in neonatal outcomes after intratracheal administration of combination budesonide/surfactant (ITBS)in infants at risk of bronchopulmonary dysplasia. However, the effect of ITBSon lung function and alveolar structure is not known. We aimed to determine the effect of ITBSon lung function, parenchymal structure and inflammatory cytokine expression in a relevant preterm animal model for bronchopulmonary dysplasia. Premature neonatal rabbits were administered a single dose of ITBSon the day of delivery and exposed to 95% oxygen. Following seven days of hyperoxia,in vivoforced oscillation and pressure-volume maneuvers were performed to examine pulmonary function. Histological and molecular analysis was performed to assess alveolar and extracellular matrix (ECM) morphology, along with gene expression of connective tissue growth factor (CTGF), IL-8 and CCL-2. ITBS attenuated the functional effect of hyperoxia-induced lung injury and limited the change to respiratory system impedance, measured using the forced oscillation technique. Treatment effects were most obvious in the small airways, with significant effects on small airway resistance and reactance. Additionally, ITBS mitigated the decrease of inspiratory capacity and static compliance. ITBS restricted alveolar septal thickening without altering the mean linear intercept and mitigated hyperoxia-induced remodeling of the ECM. These structural changes were associated with improved inspiratory capacity and lung compliance. Gene expression of CTGF IL-8 and CCL-2 were significantly down regulated in the lung. Treatment with ITBS shortly after delivery attenuated the functional and structural consequences of hyperoxia-induced lung injury to day 7 of life in the preterm rabbit.

Jaar van publicatie:2020

BOF-keylabel:ja

IOF-keylabel:ja

BOF-publication weight:3

CSS-citation score:2

Auteurs:International

Authors from:Higher Education

Toegankelijkheid:Open