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The independent biological activity of Bacillus thuringiensis Cry23Aa protein against Cylas puncticollis

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The Cry23Aa/Cry37Aa proteins fromBacillus thuringiensis(Bt) have been described toxic toCylas puncticollislarvae. In general, it is believed that Cry23Aa and Cry37Aa act jointly to exert the insecticidal activity, while there is no evidence of their toxicity individually. Therefore, in the present study, the contribution of each protein in the insecticidal activity towardC. puncticollislarvae has been assessed. The results showed that both proteins were toxic forC. puncticollislarvae when tested individually. Contrary to what was claimed previously, our results suggest that the presence of both proteins is not necessary to exert toxicity againstC. puncticollislarvae. Also, the binding behavior of Cry23Aa protein to midgut receptors ofC. puncticollislarvae has been determined. According to our results, Cry23Aa binds toC. puncticollisbrush border membrane vesicles (BBMV) specifically and independently of Cry37Aa. Due to the lack of common binding sites, Cry23Aa can be pyramided with Cry3Aa protein for better management ofC. puncticollis.
Tijdschrift: FRONTIERS IN MICROBIOLOGY
ISSN: 1664-302X
Volume: 11
Jaar van publicatie:2020
Toegankelijkheid:Open