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Electroporation loading and dye transfer : a safe and robust method to probe gap junctional coupling

Boekbijdrage - Hoofdstuk

Intercellular communication occurring via gap junction channels is considered a key mechanism for synchronizing physiological functions of cells and for the maintenance of tissue homeostasis. Gap junction channels are protein channels that are situated between neighboring cells and that provide a direct, yet selective route for the passage of small hydrophilic biomolecules and ions. Here, an electroporation method is described to load a localized area within an adherent cell monolayer with a gap junction-permeable fl uorescent reporter dye. The technique results in a rapid and effi cient labeling of a small patch of cells within the cell culture, without affecting cellular viability. Dynamic and quantitative information on gap junctional communication can subsequently be extracted by tracing the intercellular movement of the dye via time-lapse microscopy.
Boek: Gap junction protocols
Series: Methods in Molecular Biology
Pagina's: 155 - 169
ISBN:9781493936649
Toegankelijkheid:Closed