Reversal of hyperglycaemia in diabetic mice by a marginal islet mass together with human blood outgrowth endothelial cells is independent of the delivery technique and blood clot-induced processes

We recently reported that human blood outgrowth endothelial cells (BOEC) are
supportive to reverse hyperglycaemia in marginal islet mass-transplanted
diabetic mice. In this report we investigated whether the observed effect was
evoked by islet packing in a blood clot prior to transplantation or could be
mimicked by another method of islet/cell delivery.
A marginal islet mass with or without BOEC was grafted underneath the kidney
capsule of diabetic recipient mice via a (blood clot-independent) tubing system
and compared to previous islet packing in a blood clot. The effect on metabolic
outcome of both delivery techniques as well as the additive effect of BOEC was
subsequently evaluated. Marginal islet mass transplantation via a tubing system
required more islets per recipient than via a blood clot. Using the tubing
method, transplantation of a marginal islet mass combined with 5x10E5 BOEC resulted in reversal of hyperglycaemia, improved glucose tolerance and increased kidney insulin content.
The present study provides evidence that (i) previous packing in a blood clot
results in more effective islet delivery compared to tubing; (ii) BOEC exert a
beneficial effect on marginal islet transplantation, independent of grafting
technique and potential blood clot-induced processes. These data further
support the use of BOEC in (pre-) clinical studies that aim to improve current
islet transplantation protocols.

Jaar van publicatie:2013

Trefwoorden:Animals, Blood Coagulation, Blood Glucose/metabolism, Diabetes Mellitus, Experimental/surgery, Endothelial Cells/transplantation, Humans, Hyperglycemia/surgery, Islets of Langerhans/physiology, Islets of Langerhans Transplantation/methods, Male, Mice, Mice, Inbred NOD, Mice, SCID

CSS-citation score:1

Toegankelijkheid:Closed