Endometrial gene expression in the early luteal phase is impacted by mode of triggering final oocyte maturation in recFSH stimulated and GnRH antagonist co-treated IVF cycles

study question: Do differences in endometrial gene expression exist after ovarian stimulation with four different regimens of
triggering final oocyte maturation and luteal phase support in the same patient?
summary answer: Significant differences in the expression of genes involved in receptivity and early implantation were seen between
the four protocols.
what is known already: GnRH agonist triggering is an alternative to hCG triggering in GnRH antagonist co-treated cycles, resulting
in an elimination of early ovarian hyperstimulation syndrome. Whereas previous studies have revealed a low ongoing clinical pregnancy
rate after GnRH agonist trigger due to a high early pregnancy loss rate, despite supplementation with the standard luteal phase support, more
recent studies, employing a 'modified' luteal phase support including a bolus of 1500 IU hCG on the day of oocyte aspiration, have reported
ongoing pregnancy rates similar to those seen after hCG triggering.
study design, size duration: A prospective randomized study was performed in four oocyte donors recruited from an oocyte
donation program during the period 2010-2011.
participants, materials, setting, methods: Four oocyte donors in a university IVF center each prospectively underwent
four consecutive stimulation protocols, with different modes of triggering final oocyte maturation and a different luteal phase
support, followed by endometrial biopsy on Day 5 after oocyte retrieval. The following protocols were used: (A) 10 000 IU hCG and standard
luteal phase support, (B) GnRH agonist (triptorelin 0.2 mg), followed by 1500 IU hCG 35 h after triggering final oocyte maturation, and
standard luteal phase support, (C) GnRH agonist (triptorelin 0.2 mg) and standard luteal phase support and (D) GnRH agonist (triptorelin
0.2 mg) without luteal phase support. Microarray data analysis was performed with GeneSpring GX 11.5 (RMA algorithm). Pathway and
network analysis was performed with the gene ontology software Ingenuity Pathways Analysis (Ingenuityw Systems, www.ingenuity.com,
Redwood City, CA, USA). Samples were grouped and background intensity values were removed (cutoff at the lowest 20th percentile).
A one-way ANOVA test (P , 0.05) was performed with Benjamini-Hochberg multiple testing correction.
main results: Significant differences were seen in endometrial gene expression, related to the type of ovulation trigger and luteal phase
support. However, the endometrial gene expression after the GnRH agonist trigger and a modified luteal phase support (B) was similar to the
pattern seen after the hCG trigger (A).
limitations, reasons for caution: The study was performed in four oocyte donors only; however, it is a strength of the
study that the same donor underwent four consecutive stimulation protocols within 1 year to avoid inter-individual variations.

Jaar van publicatie:2012

Trefwoorden:gene expression, agonist trigger