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Evaluation of the multipotent character of human adipose derived stem cells isolated by Ficoll gradient centrifugation and red blood cell lysis treatment
Tijdschriftbijdrage - Tijdschriftartikel
In the present study, the multipotent potential of two differently isolated human adipose derived stem cell (hADSC) populations was evaluated. More specifically, hADSC isolated by means of classical ficoll (F) gradient centrifugation were compared to hADSC isolated by means of red blood cell (RBC) lysis and subsequent cultivation as 3D spheres.
No significant difference in the genotypic expression of the multipotent markers octamer binding transcription factor (Oct) 4, sex determining region-Y box (Sox) 2, Nanog, Krüppel-like factor (Klf) 4 and cMyc could be observed between the two isolation methods. Upon adipogenic and osteogenic differentiation, both hADSC populations showed lipid droplet accumulation and mineral deposition, respectively. Although, a more pronounced mineral deposition was observed in hADSC isolated by RBC, suggesting a higher osteogenic potential. Upon exposure to keratinogenic media, both hADSC populations expressed the keratinocyte markers filaggrin and involucrin evidencing a successful keratinogenic differentiation. However, no differences in expression were observed between the two different isolation procedures. Finally, upon exposure to neurogenic media, a significant difference in marker expression was observed. Indeed, hADSC-RBC only expressed vimentin and nestin. In contrast, hADSC-F expressed vimentin, nestin, neurofilament (NF) 200, myelin basic protein (MBP) and tyrosine hydroxylase (TH), suggesting a higher neurogenic potential.
In summary, our data suggest that the most efficient isolation procedure in terms of differentiation capacity depends on the desired cell type.
No significant difference in the genotypic expression of the multipotent markers octamer binding transcription factor (Oct) 4, sex determining region-Y box (Sox) 2, Nanog, Krüppel-like factor (Klf) 4 and cMyc could be observed between the two isolation methods. Upon adipogenic and osteogenic differentiation, both hADSC populations showed lipid droplet accumulation and mineral deposition, respectively. Although, a more pronounced mineral deposition was observed in hADSC isolated by RBC, suggesting a higher osteogenic potential. Upon exposure to keratinogenic media, both hADSC populations expressed the keratinocyte markers filaggrin and involucrin evidencing a successful keratinogenic differentiation. However, no differences in expression were observed between the two different isolation procedures. Finally, upon exposure to neurogenic media, a significant difference in marker expression was observed. Indeed, hADSC-RBC only expressed vimentin and nestin. In contrast, hADSC-F expressed vimentin, nestin, neurofilament (NF) 200, myelin basic protein (MBP) and tyrosine hydroxylase (TH), suggesting a higher neurogenic potential.
In summary, our data suggest that the most efficient isolation procedure in terms of differentiation capacity depends on the desired cell type.
Tijdschrift: Toxicology in Vitro
ISSN: 0887-2333
Issue: 25
Volume: 6
Pagina's: 1224-1230
Jaar van publicatie:2011
Trefwoorden:human adipose tissue-derived stem cells, stem cell plasticity, stem cell multipotency, isolation of adult stem cells, red blood cell lysis