Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene encoding a nitronate monooxygenase

The PA4203 gene encodes a LysR regulator and lies between the ppgL gene (PA4204), which encodes a periplasmic gluconolactonase, and in the opposite orientation the PA4202 nmoA gene coding for a nitronate monooxygenase and ddlA (PA4201) encoding a D-alanine alanine ligase. The intergenic regions between PA4203 and ppgL, and nmoA are very short (79 and 107 nucleotides, respectively). Here we show that PA4203 (nmoR) represses its own transcription and the expression of nmoA. A chromatin immunoprecipation analysis showed the presence of a single NmoR binding site between nmoA and nmoR, which was confirmed by Electrophoretic mobility shift assays (EMSAs) with the purified NmoR protein. Despite this, a transcriptome analysis revealed more genes to be affected in an nmoR mutant, including genes known to be part of the MexT LysR activator regulon. The PA1225 gene encoding a quinone oxidoreductase is the most highly up-regulated in the nmoR deletion mutant, independently of MexT. Finally, deletion of the nmoA gene resulted in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), confirming the role of nitronate monooxygenase protein in the detoxification of nitronate

Jaar van publicatie:2014

Trefwoorden:gluconolactonase

CSS-citation score:1

Toegankelijkheid:Open