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Publicatie
Genetic lineage tracing of beta cell neogenesis
Boekbijdrage - Hoofdstuk
Genetic lineage tracing is an invaluable tool to demonstrate and measure neogenesis of beta cells from putative precursor cells. Cre-Lox recombination technology can be used for indelible labelling of a cohort of cells and following the fate of these cells and their progeny in animal models. Here, the combination is described of beta-galactosidase enzymatic staining with immunohistochemical staining to demonstrate labelled cells. This technique is performed in tissue cryosections.
Boek: Animal models in diabetes research
Series: Methods in Molecular Biology
Pagina's: 317-322
Aantal pagina's: 6
ISBN:978-1-62703-067-0
Jaar van publicatie:2012
Trefwoorden:diabetes