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Progesterone rise on the hCG day in GnRH antagonist/rec-FSH stimulated cycles affects endometrial gene expression
Tijdschriftbijdrage - Tijdschriftartikel
Premature progesterone rise during GnRH-antagonist cycles for IVF is a frequent
phenomenon and has been associated with lower pregnancy and implantation rates. In this study, we
evaluated endometrial gene expression on the day of oocyte retrieval according to the level of serum P
on the day of hCG administration in GnRH-antagonist/rec-FSH IVF cycles with fresh embryo transfer.
Endometrial biopsies (n=14) were analysed with Affymetrix HG U133 Plus2.0 Arrays. Patients were
divided into three groups according to their P serum concentration on the day of hCG administration:
(A) P 1.5 ng/mL. Gene expression analysis showed
a small number of significantly differentially expressed probe sets between groups A and B (5 up/23
down in B) and a large difference between groups B and C (607 up/212 down) (P=1.4).
Validation was performed with QPCR on selected genes. This is the first study to demonstrate a distinct
difference in endometrial gene expression profile between patients with a P serum level above and
below the threshold of 1.5 ng/mL on the day of hCG administration, which might reflect an important
issue for implantation failure in IVF patients with premature progesterone rise.
phenomenon and has been associated with lower pregnancy and implantation rates. In this study, we
evaluated endometrial gene expression on the day of oocyte retrieval according to the level of serum P
on the day of hCG administration in GnRH-antagonist/rec-FSH IVF cycles with fresh embryo transfer.
Endometrial biopsies (n=14) were analysed with Affymetrix HG U133 Plus2.0 Arrays. Patients were
divided into three groups according to their P serum concentration on the day of hCG administration:
(A) P 1.5 ng/mL. Gene expression analysis showed
a small number of significantly differentially expressed probe sets between groups A and B (5 up/23
down in B) and a large difference between groups B and C (607 up/212 down) (P=1.4).
Validation was performed with QPCR on selected genes. This is the first study to demonstrate a distinct
difference in endometrial gene expression profile between patients with a P serum level above and
below the threshold of 1.5 ng/mL on the day of hCG administration, which might reflect an important
issue for implantation failure in IVF patients with premature progesterone rise.
Tijdschrift: Reproductive Biomedicine Online
ISSN: 1472-6483
Volume: 22
Pagina's: 263-271
Jaar van publicatie:2011
Trefwoorden:implantation, progesterone, gene expression